{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:00:27Z","timestamp":1789783227694,"version":"4.0.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            2D6 is a dimeric monoclonal immunoglobulin A (IgA) specific for the nonreducing terminal residue of Ogawa O-polysaccharide (OPS) of\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            . It was previously demonstrated that 2D6 IgA is sufficient to passively protect suckling mice from oral challenge with virulent\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            O395. In this study, we sought to define the mechanism by which 2D6 IgA antibody protects the intestinal epithelium from\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            infection. In a mouse ligated-ileal-loop assay, 2D6 IgA promoted\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            agglutination in the intestinal lumen and limited the ability of the bacteria to associate with the epithelium, particularly within the crypt regions.\n            <jats:italic>In vitro<\/jats:italic>\n            fluorescence digital video microscopy analysis of antibody-treated\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            in liquid medium revealed that 2D6 IgA not only induced the rapid (5- to 10-min) onset of agglutination but was an equally potent inhibitor of bacterial motility. Scanning electron microscopy showed that 2D6 IgA promoted flagellum-flagellum cross-linking, as well as flagellar entanglement with bacterial bodies, suggesting that motility arrest may be a consequence of flagellar tethering. However, monovalent 2D6 Fab fragments also inhibited\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            motility, demonstrating that antibody-mediated agglutination and motility arrest are separate phenomena. While 2D6 IgA is neither bactericidal nor bacteriostatic, exposure of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            to 2D6 IgA (or Fab fragments) resulted in a 5-fold increase in surface-associated blebs, as well an onset of a wrinkled surface morphotype. We propose that the protective immunity conferred by 2D6 IgA is the result of multifactorial effects on\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            , including agglutination, motility arrest, and possibly outer membrane stress.\n          <\/jats:p>","DOI":"10.1128\/iai.02856-14","type":"journal-article","created":{"date-parts":[[2015,2,10]],"date-time":"2015-02-10T06:36:38Z","timestamp":1423550198000},"page":"1674-1683","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Rapid Effects of a Protective O-Polysaccharide-Specific Monoclonal IgA on Vibrio cholerae Agglutination, Motility, and Surface Morphology"],"prefix":"10.1128","volume":"83","author":[{"given":"Kara J.","family":"Levinson","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA"},{"name":"Department of Biomedical Sciences, University at Albany, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdia","family":"De Jesus","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5083-8640","authenticated-orcid":false,"given":"Nicholas J.","family":"Mantis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA"},{"name":"Department of Biomedical Sciences, University at Albany, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60436-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60560-1"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00543-12"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.11.5.890-897.1975"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111581598"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004405"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-01846-6_2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03546.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.10.150"},{"key":"e_1_3_3_11_2","first-page":"309","article-title":"Notes from the field: identification of Vibrio cholerae serogroup O1, serotype Inaba, biotype El Tor strain-Haiti, March 2012","volume":"61","author":"Centers for Disease Control and Prevention","year":"2012","unstructured":"Centers for Disease Control and Prevention. 2012. Notes from the field: identification of Vibrio cholerae serogroup O1, serotype Inaba, biotype El Tor strain-Haiti, March 2012. MMWR Morb Mortal Wkly Rep 61:309.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.12.5279-5285.1993"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.3.977-982.1991"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00398-10"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01382-12"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.32.1.1-8.1981"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.32.1.9-14.1981"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000221"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00037-12"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00481-10"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3109\/08820131003622635"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00813-12"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00042-11"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00416-08"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00018-12"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.5.2777"},{"key":"e_1_3_3_27_2","volume-title":"Abstr 114th Gen Meet Am Soc Microbiol American Society for Microbiology","author":"Levinson K","year":"2014","unstructured":"Levinson K, Koestler B, Waters C, Mantis N. 2014. Protective anti-LPS monoclonal antibodies in Vibrio cholerae, abstr D-2094. Abstr 114th Gen Meet Am Soc Microbiol American Society for Microbiology, Washington, DC. http:\/\/gm.asm.org."},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50491-9"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00051-6"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.5.1786-1792.1992"},{"key":"e_1_3_3_31_2","first-page":"42","article-title":"Selective adhesion of IgA to murine intestinal M cells","volume":"69","author":"Mantis NJ","year":"1999","unstructured":"Mantis NJ, Cheung MC, Neutra MR. 1999. Selective adhesion of IgA to murine intestinal M cells. Immunol Lett 69:42.","journal-title":"Immunol Lett"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.3791\/50167"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1995.tb03260.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802241105"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00321-12"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2011.41"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00341-2"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.177.4050.697"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02013655"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.16.5104-5110.1997"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073413"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01764.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00378-11"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2556"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.060022997"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.02856-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.02856-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:58:55Z","timestamp":1646474335000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.02856-14"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1128\/IAI.02856-14"],"URL":"https:\/\/doi.org\/10.1128\/iai.02856-14","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"2014-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}