{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T14:32:43Z","timestamp":1793370763064,"version":"4.5.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Lyme disease spirochete\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            is dependent on purine salvage from the host environment for survival. The genes\n            <jats:italic>bbb22<\/jats:italic>\n            and\n            <jats:italic>bbb23<\/jats:italic>\n            encode purine permeases that are essential for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            mouse infectivity. We now demonstrate the unique contributions of each of these genes to purine transport and murine infection. The affinities of spirochetes carrying\n            <jats:italic>bbb22<\/jats:italic>\n            alone for hypoxanthine and adenine were similar to those of spirochetes carrying both genes. Spirochetes carrying\n            <jats:italic>bbb22<\/jats:italic>\n            alone were able to achieve wild-type levels of adenine saturation but not hypoxanthine saturation, suggesting that maximal hypoxanthine uptake requires the presence of\n            <jats:italic>bbb23<\/jats:italic>\n            . Moreover, the purine transport activity conferred by\n            <jats:italic>bbb22<\/jats:italic>\n            was dependent on an additional distal transcriptional start site located within the\n            <jats:italic>bbb23<\/jats:italic>\n            open reading frame. The initial rates of uptake of hypoxanthine and adenine by spirochetes carrying\n            <jats:italic>bbb23<\/jats:italic>\n            alone were below the level of detection. However, these spirochetes demonstrated a measurable increase in hypoxanthine uptake over a 30-min time course. Our findings indicate that\n            <jats:italic>bbb22<\/jats:italic>\n            -dependent adenine transport is essential for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            survival in mice. The\n            <jats:italic>bbb23<\/jats:italic>\n            gene was dispensable for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            mouse infectivity, yet its presence was required along with that of\n            <jats:italic>bbb22<\/jats:italic>\n            for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            to achieve maximal spirochete loads in infected mouse tissues. These data demonstrate that both genes,\n            <jats:italic>bbb22<\/jats:italic>\n            and\n            <jats:italic>bbb23<\/jats:italic>\n            , are critical for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            to achieve wild-type infection of mice and that the differences in the capabilities of the two transporters may reflect distinct purine salvage needs that the spirochete encounters throughout its natural infectious cycle.\n          <\/jats:p>","DOI":"10.1128\/iai.02859-14","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T02:12:30Z","timestamp":1426558350000},"page":"2224-2233","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Molecular Dissection of a Borrelia burgdorferi\n            <i>In Vivo<\/i>\n            Essential Purine Transport System"],"prefix":"10.1128","volume":"83","author":[{"given":"Sunny","family":"Jain","sequence":"first","affiliation":[{"name":"Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne C.","family":"Showman","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mollie W.","family":"Jewett","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, 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