{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:41:11Z","timestamp":1790674871273,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enterotoxigenic\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            (ETEC), a leading cause of acute diarrhea, colonizes the intestine by means of adhesins. However, 15 to 50% of clinical isolates are negative for known adhesins, making it difficult to identify antigens for broad-coverage vaccines. The ETEC strain 1766a, obtained from a child with watery diarrhea in Chile, harbors the colonization factor CS23 but is negative for other known adhesins. One clone, derived from an ETEC 1766a genomic library (clone G10), did not produce CS23 yet was capable of adhering to Caco-2 cells. The goal of this study was to identify the gene responsible for this capacity. Random transposon-based mutagenesis allowed the identification of a 4,110-bp gene that codes for a homologue of the temperature-sensitive hemagglutinin (Tsh) autotransporter described in avian\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            strains (97% identity, 90% coverage) and that is called TleA (Tsh-like ETEC autotransporter) herein. An isogenic ETEC 1766a strain with a\n            <jats:italic>tleA<\/jats:italic>\n            mutation showed an adhesion level similar to that of the wild-type strain, suggesting that the gene does not direct attachment to Caco-2 cells. However, expression of\n            <jats:italic>tleA<\/jats:italic>\n            conferred the capacity for adherence to nonadherent\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            HB101. This effect coincided with the detection of TleA on the surface of nonpermeabilized bacteria, while, conversely, ETEC 1766a seems to secrete most of the produced autotransporter to the medium. On the other hand, TleA was capable of degrading bovine submaxillary mucin and leukocyte surface glycoproteins CD45 and P-selectin glycoprotein ligand 1 (PSGL-1). These results suggest that TleA promotes colonization of the intestinal epithelium and that it may modulate the host immune response.\n          <\/jats:p>","DOI":"10.1128\/iai.02976-14","type":"journal-article","created":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T12:53:06Z","timestamp":1424955186000},"page":"1893-1903","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["TleA, a Tsh-Like Autotransporter Identified in a Human Enterotoxigenic Escherichia coli Strain"],"prefix":"10.1128","volume":"83","author":[{"given":"Daniela","family":"Guti\u00e9rrez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Qu\u00edmica y Biolog\u00eda, Universidad de Santiago de Chile, Santiago, Chile"},{"name":"Programa de Microbiolog\u00eda y Micolog\u00eda, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirka","family":"Pardo","sequence":"additional","affiliation":[{"name":"Programa de Microbiolog\u00eda y Micolog\u00eda, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Montero","sequence":"additional","affiliation":[{"name":"Programa de Microbiolog\u00eda y Micolog\u00eda, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"O\u00f1ate","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio J.","family":"Farf\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Estudios Moleculares, Departamento de Pediatr\u00eda, Hospital Dr. Luis Calvo Mackenna, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Ruiz-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Del Canto","sequence":"additional","affiliation":[{"name":"Programa de Microbiolog\u00eda y Micolog\u00eda, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Vidal","sequence":"additional","affiliation":[{"name":"Programa de Microbiolog\u00eda y Micolog\u00eda, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"97","article-title":"Future directions for research on enterotoxigenic Escherichia coli vaccines for developing countries","volume":"81","author":"WHO","year":"2006","unstructured":"WHO. 2006. 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