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The Cpx envelope stress response, composed of the sensor histidine kinase CpxA and the response regulator CpxR, senses and mediates adaptation to insults to the bacterial envelope. The Cpx response has been implicated in the regulation of a number of envelope-localized virulence determinants across bacterial species. Here, we show that activation of the Cpx pathway in\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            El Tor strain C6706 leads to a decrease in expression of the major virulence factors in this organism, cholera toxin (CT) and the toxin-coregulated pilus (TCP). Our results indicate that this occurs through the repression of production of the ToxT regulator and an additional upstream transcription factor, TcpP. The effect of the Cpx response on CT and TCP expression is mostly abrogated in a cyclic AMP receptor protein (CRP) mutant, although expression of the\n            <jats:italic>crp<\/jats:italic>\n            gene is unaltered. Since TcpP production is controlled by CRP, our data suggest a model whereby the Cpx response affects CRP function, which leads to diminished TcpP, ToxT, CT, and TCP production.\n          <\/jats:p>","DOI":"10.1128\/iai.03056-14","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T09:46:18Z","timestamp":1427881578000},"page":"2396-2408","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["The Cpx System Regulates Virulence Gene Expression in Vibrio cholerae"],"prefix":"10.1128","volume":"83","author":[{"given":"Nicole","family":"Acosta","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Pukatzki","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy L.","family":"Raivio","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1128\/9781555818364","volume-title":"Vibrio cholerae and cholera: molecular to global perspectives","author":"Wachsmuth IK","year":"1994","unstructured":"Wachsmuth IK, Olsvik \u00d8, Evins GM, Popovic T. 1994. 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