{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:13:33Z","timestamp":1790032413171,"version":"4.0.1"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Gram-negative bacterium and opportunistic pathogen\n            <jats:named-content content-type=\"genus-species\">Serratia marcescens<\/jats:named-content>\n            causes ocular infections in healthy individuals. Secreted protease activity was characterized from 44 ocular clinical isolates, and a higher frequency of protease-positive strains was observed among keratitis isolates than among conjunctivitis isolates. A positive correlation between protease activity and cytotoxicity to human corneal epithelial cells\n            <jats:italic>in vitro<\/jats:italic>\n            was determined. Deletion of\n            <jats:italic>prtS<\/jats:italic>\n            in clinical keratitis isolate K904 reduced, but did not eliminate, cytotoxicity and secreted protease production. This indicated that PrtS is necessary for full cytotoxicity to ocular cells and implied the existence of another secreted protease(s) and cytotoxic factors. Bioinformatic analysis of the\n            <jats:named-content content-type=\"genus-species\">S. marcescens<\/jats:named-content>\n            Db11 genome revealed three additional open reading frames predicted to code for serralysin-like proteases noted here as\n            <jats:italic>slpB<\/jats:italic>\n            ,\n            <jats:italic>slpC<\/jats:italic>\n            , and\n            <jats:italic>slpD<\/jats:italic>\n            . Induced expression of\n            <jats:italic>prtS<\/jats:italic>\n            and\n            <jats:italic>slpB<\/jats:italic>\n            , but not\n            <jats:italic>slpC<\/jats:italic>\n            and\n            <jats:italic>slpD<\/jats:italic>\n            , in strain PIC3611 rendered the strain cytotoxic to a lung carcinoma cell line; however, only\n            <jats:italic>prtS<\/jats:italic>\n            induction was sufficient for cytotoxicity to a corneal cell line. Strain K904 with deletion of both\n            <jats:italic>prtS<\/jats:italic>\n            and\n            <jats:italic>slpB<\/jats:italic>\n            genes was defective in secreted protease activity and cytotoxicity to human cell lines. PAGE analysis suggests that SlpB is produced at lower levels than PrtS. Purified SlpB demonstrated calcium-dependent and AprI-inhibited protease activity and cytotoxicity to airway and ocular cell lines\n            <jats:italic>in vitro<\/jats:italic>\n            . Lastly, genetic analysis indicated that the type I secretion system gene,\n            <jats:italic>lipD<\/jats:italic>\n            , is required for SlpB secretion. These genetic data introduce SlpB as a new cytotoxic protease from\n            <jats:named-content content-type=\"genus-species\">S. marcescens<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.03096-14","type":"journal-article","created":{"date-parts":[[2015,5,5]],"date-time":"2015-05-05T01:41:12Z","timestamp":1430790072000},"page":"2907-2916","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Identification of SlpB, a Cytotoxic Protease from Serratia marcescens"],"prefix":"10.1128","volume":"83","author":[{"given":"Robert M. Q.","family":"Shanks","sequence":"first","affiliation":[{"name":"The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"},{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Stella","sequence":"additional","affiliation":[{"name":"The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin M.","family":"Hunt","sequence":"additional","affiliation":[{"name":"The Charles T. 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