{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:42:24Z","timestamp":1790757744843,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ability of the human bacterial pathogen\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            to cause invasive disease depends on survival in the bloodstream via mechanisms to suppress complement activation. In this study, we show that prophage genes coding for T and B cell stimulating protein B (TspB), which is an immunoglobulin-binding protein, are essential for survival of\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            group B strain H44\/76 in normal human serum (NHS). H44\/76 carries three genes coding for TspB. Mutants having all\n            <jats:italic>tspB<\/jats:italic>\n            genes inactivated did not survive in &gt;5% NHS or IgG-depleted NHS. TspB appeared to inhibit IgM-mediated activation of the classical complement pathway, since survival of the\n            <jats:italic>tspB<\/jats:italic>\n            triple knockout was the same as that of the parent strain or a complemented mutant when the classical pathway was inactivated by depleting NHS of C1q and was increased in IgM-depleted NHS. A mutant solely carrying\n            <jats:italic>tspB<\/jats:italic>\n            gene\n            <jats:italic>nmbh4476_0681<\/jats:italic>\n            was as resistant as the parent strain, while mutants carrying only\n            <jats:italic>nmbh4476_0598<\/jats:italic>\n            or\n            <jats:italic>nmbh4476_1698<\/jats:italic>\n            were killed in \u22655% NHS. The phenotype associated with TspB is formation of a matrix containing TspB, IgG, and DNA that envelopes aggregates of bacteria. Recombinant proteins corresponding to particular subdomains of TspB were found to have human IgG Fc\u03b3- and\/or DNA-binding activity, but only TspB derivatives containing both domains formed large, biofilm-like aggregates when combined with purified IgG and DNA. Recognizing the role of TspB in serum resistance may lead to a better understanding of why strains that carry\n            <jats:italic>tspB<\/jats:italic>\n            genes are associated with invasive meningococcal disease.\n          <\/jats:p>","DOI":"10.1128\/iai.03134-14","type":"journal-article","created":{"date-parts":[[2015,1,13]],"date-time":"2015-01-13T05:18:40Z","timestamp":1421126320000},"page":"1257-1264","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Resistance of Neisseria meningitidis to Human Serum Depends on T and B Cell Stimulating Protein B"],"prefix":"10.1128","volume":"83","author":[{"given":"Maike G.","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"UCSF Benioff Children's Hospital Oakland Research Institute, Oakland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina E.","family":"Moe","sequence":"additional","affiliation":[{"name":"UCSF Benioff Children's Hospital Oakland Research Institute, Oakland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip Q.","family":"Richards","sequence":"additional","affiliation":[{"name":"UCSF Benioff Children's Hospital Oakland Research Institute, Oakland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory R.","family":"Moe","sequence":"additional","affiliation":[{"name":"UCSF Benioff Children's Hospital Oakland Research Institute, Oakland, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.46.2.162-190.1982"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45529-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61016-2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050112"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301153"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020552"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1708-1714.1999"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7293-7203.2001"},{"key":"e_1_3_3_10_2","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council","year":"2011","unstructured":"National Research Council. 2011. 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