{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T01:43:43Z","timestamp":1789436623040,"version":"build-2803163510"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Rickettsia rickettsii<\/jats:named-content>\n                    is an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. Strains of\n                    <jats:named-content content-type=\"genus-species\">R. rickettsii<\/jats:named-content>\n                    differ dramatically in virulence. In a guinea pig model of infection, the severity of disease as assessed by fever response varies from the most virulent, Sheila Smith, to Iowa, which causes no fever. To identify potential determinants of virulence in\n                    <jats:named-content content-type=\"genus-species\">R. rickettsii<\/jats:named-content>\n                    , the genomes of two additional strains were sequenced for comparison to known sequences (comparative genome sequencing [CGS]).\n                    <jats:named-content content-type=\"genus-species\">R. rickettsii<\/jats:named-content>\n                    Morgan and R strains were compared to the avirulent\n                    <jats:named-content content-type=\"genus-species\">R. rickettsii<\/jats:named-content>\n                    Iowa and virulent\n                    <jats:named-content content-type=\"genus-species\">R. rickettsii<\/jats:named-content>\n                    Sheila Smith strains. The Montana strains Sheila Smith and R were found to be highly similar while the eastern strains Iowa and Morgan were most similar to each other. A major surface antigen, rickettsial outer membrane protein A (rOmpA), is severely truncated in the Iowa strain. The region of\n                    <jats:italic>ompA<\/jats:italic>\n                    containing 13 tandem repeats was sequenced, revealing only seven shared SNPs (four nonsynonymous) for R and Morgan strains compared to Sheila Smith, with an additional 17 SNPs identified in Morgan. Another major surface antigen and autotransporter, rOmpB, exhibits a defect in processing in the Iowa strain such that the beta fragment is not cleaved. Sequence analysis of\n                    <jats:italic>ompB<\/jats:italic>\n                    reveals identical sequences between Iowa and Morgan strains and between the R and Sheila Smith strains. The number of SNPs and insertions\/deletions between sequences of the two Montana strains and the two eastern strains is low, thus narrowing the field of possible virulence factors.\n                  <\/jats:p>","DOI":"10.1128\/iai.03140-14","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T02:53:10Z","timestamp":1422931990000},"page":"1568-1576","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Comparative Genome Sequencing of Rickettsia rickettsii Strains That Differ in Virulence"],"prefix":"10.1128","volume":"83","author":[{"given":"Tina R.","family":"Clark","sequence":"first","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas F.","family":"Noriea","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DeAnna C.","family":"Bublitz","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damon W.","family":"Ellison","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Martens","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika I.","family":"Lutter","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted","family":"Hackstadt","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"843","article-title":"Some aspects of Rocky Mountain spotted fever as shown by recent investigation","volume":"76","author":"Ricketts HT","year":"1909","unstructured":"Ricketts HT. 1909. 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