{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:41:15Z","timestamp":1791186075972,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            , the cause of serious and life-threatening diseases in humans, is of national biodefense concern because of its potential use as a bioterrorism agent. This microbe is listed as a select agent by the CDC; therefore, development of vaccines is of significant importance. Here, we further investigated the growth characteristics of a recently created\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            1026b \u0394\n            <jats:italic>asd<\/jats:italic>\n            mutant\n            <jats:italic>in vitro<\/jats:italic>\n            , in a cell model, and in an animal model of infection. The mutant was typified by an inability to grow in the absence of exogenous diaminopimelate (DAP); upon single-copy complementation with a wild-type copy of the\n            <jats:italic>asd<\/jats:italic>\n            gene, growth was restored to wild-type levels. Further characterization of the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            \u0394\n            <jats:italic>asd<\/jats:italic>\n            mutant revealed a marked decrease in RAW264.7 murine macrophage cytotoxicity compared to the wild type and the complemented \u0394\n            <jats:italic>asd<\/jats:italic>\n            mutant. RAW264.7 cells infected by the \u0394\n            <jats:italic>asd<\/jats:italic>\n            mutant did not exhibit signs of cytopathology or multinucleated giant cell (MNGC) formation, which were observed in wild-type\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            cell infections. The \u0394\n            <jats:italic>asd<\/jats:italic>\n            mutant was found to be avirulent in BALB\/c mice, and mice vaccinated with the mutant were protected against acute inhalation melioidosis. Thus, the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. pseudomallei<\/jats:named-content>\n            \u0394\n            <jats:italic>asd<\/jats:italic>\n            mutant may be a promising live attenuated vaccine strain and a biosafe strain for consideration of exclusion from the select agent list.\n          <\/jats:p>","DOI":"10.1128\/iai.05044-11","type":"journal-article","created":{"date-parts":[[2011,8,2]],"date-time":"2011-08-02T02:57:50Z","timestamp":1312253870000},"page":"4010-4018","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["The Burkholderia pseudomallei \u0394\n            <i>asd<\/i>\n            Mutant Exhibits Attenuated Intracellular Infectivity and Imparts Protection against Acute Inhalation Melioidosis in Mice"],"prefix":"10.1128","volume":"79","author":[{"given":"Michael H.","family":"Norris","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Manoa, Hawaii 96822"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie L.","family":"Propst","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hawaii at Manoa, Manoa, Hawaii 96822"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven W.","family":"Dow","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert P.","family":"Schweizer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tung T.","family":"Hoang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hawaii at Manoa, Manoa, Hawaii 96822"},{"name":"Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Manoa, Hawaii 96822"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-7-539"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00531-08"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901466"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(08)70022-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.10.018"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00263-08"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02430-07"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01240-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.1.52"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.7.2116-2125.1997"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-0344(00)00072-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90464-3"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01384-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/508217"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/498983"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.1898-1903.1998"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90707-A"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1982.tb01178.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-3-899"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.1.584-587.2003"},{"key":"e_1_3_2_23_2","first-page":"782","article-title":"Intracellular survival of Burkholderia pseudomallei","volume":"64","author":"Jones A. 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