{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:57:51Z","timestamp":1780549071231,"version":"3.54.1"},"reference-count":92,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Borrelia burgdorferi<\/jats:named-content>\n            , the agent of Lyme disease, undergoes rapid adaptive gene expression in response to signals unique to its arthropod vector or vertebrate hosts. Among the upregulated genes under vertebrate host conditions is one of the five annotated homologs of oligopeptide permease A (OppA5, BBA34). A mutant lacking\n            <jats:italic>oppA5<\/jats:italic>\n            was constructed in an lp25-deficient isolate of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            strain B31, and the minimal regions of infectivity were restored via a shuttle vector pBBE22 with or without an intact copy of\n            <jats:italic>bba34<\/jats:italic>\n            . Immunoblot analysis of the\n            <jats:italic>bba34<\/jats:italic>\n            mutant revealed a reduction in the levels of RpoS, BosR, and CsrA\n            <jats:sub>Bb<\/jats:sub>\n            with a concomitant reduction in the levels of OspC, DbpA, BBK32, and BBA64. There were no changes in the levels of OspA, NapA, P66, and three other OppA orthologs. Quantitative transcriptional analysis correlated with the changes in the protein levels. However, the\n            <jats:italic>bba34<\/jats:italic>\n            mutant displayed comparable infectivities in the C3H\/HeN mice and the wild-type strain, despite the reduction in several pathogenesis-related proteins. Supplementation of the growth medium with increased levels of select components, notably sodium acetate and sodium bicarbonate, restored the levels of several proteins in the\n            <jats:italic>bba34<\/jats:italic>\n            mutant to wild-type levels. We speculate that the transport of acetate appears to contribute to the accumulation of key metabolites, like acetyl phosphate, that facilitate the adaptation of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            to the vertebrate host by the activation of the Rrp2-RpoN-RpoS pathway. These studies underscore the importance of solute transport to host-specific adaptation of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.05234-11","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T03:44:13Z","timestamp":1306899853000},"page":"3407-3420","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Oligopeptide Permease A5 Modulates Vertebrate Host-Specific Adaptation of Borrelia burgdorferi"],"prefix":"10.1128","volume":"79","author":[{"given":"B. V. Subba","family":"Raju","sequence":"first","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria D.","family":"Esteve-Gassent","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. L. Rajasekhar","family":"Karna","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine L.","family":"Miller","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tricia A.","family":"Van Laar","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Seshu","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3500-3509.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb01876.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00409-09"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2325"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03537.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2035-2044.2004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00036-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1222-1230.2000"},{"key":"e_1_3_2_10_2","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour A. 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