{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:13:07Z","timestamp":1770819187077,"version":"3.50.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella abortus<\/jats:named-content>\n            is a facultative intracellular bacterial pathogen that causes abortion in domestic animals and undulant fever in humans. Recent studies have revealed that Toll-like receptor (TLR)-initiated immune response to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            spp. depends on myeloid differentiation factor 88 (MyD88) signaling. Therefore, we decided to study the role of the interleukin-1 receptor-associated kinase 4 (IRAK-4) in host innate immune response against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            . After\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            infection, it was shown that the number of CFU in IRAK-4\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice was high compared to that in IRAK-4\n            <jats:sup>+\/\u2212<\/jats:sup>\n            animals only at 1 week postinfection. At 3 and 6 weeks postinfection, IRAK-4\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice were able to control the infection similarly to heterozygous animals. Furthermore, the type 1 cytokine profile was evaluated. IRAK-4\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice showed lower production of systemic interleukin-12 (IL-12) and gamma interferon (IFN-\u03b3). Additionally, a reduced percentage of CD4\n            <jats:sup>+<\/jats:sup>\n            and CD8\n            <jats:sup>+<\/jats:sup>\n            T cells expressing IFN-\u03b3 was observed compared to IRAK-4\n            <jats:sup>+\/\u2212<\/jats:sup>\n            . Further, the production of IL-12 and tumor necrosis factor alpha (TNF-\u03b1) by macrophages and dendritic cells from IRAK-4\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice was abolished at 24 h after stimulation with\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            . To investigate the role of IRAK-4 in mitogen-activated protein kinase (MAPK) and NF-\u03baB signaling pathways, macrophages were stimulated with\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            , and the signaling components were analyzed by protein phosphorylation. Extracellular signal-regulated kinase 1 (ERK1) and ERK2 and p38 as well as p65 NF-\u03baB phosphorylation was profoundly impaired in IRAK-4\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            and MyD88\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            macrophages activated by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            . In summary, the results shown in this study demonstrated that IRAK-4 is critical to trigger the initial immune response against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. abortus<\/jats:named-content>\n            but not at later phases of infection.\n          <\/jats:p>","DOI":"10.1128\/iai.05289-11","type":"journal-article","created":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T03:04:10Z","timestamp":1313463850000},"page":"4688-4695","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Interleukin-1 Receptor-Associated Kinase 4 Is Essential for Initial Host Control of Brucella abortus Infection"],"prefix":"10.1128","volume":"79","author":[{"given":"Fernanda S.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia B.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula M. 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