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Substantial progress has been made during the past 10 years toward the development of improved vaccines for brucellosis. In part, this has been achieved by the identification and characterization of live attenuated mutants that are safer in the host but still can stimulate an adequate immune response. In the present study, the identification and characterization of the<jats:italic>mucR<\/jats:italic>mutant (BMEI 1364) as a vaccine candidate for brucellosis was conducted. BALB\/c mice were vaccinated intraperitoneally at a dose of 10<jats:sup>5<\/jats:sup>CFU with the mutant to evaluate safety and protective efficacy against intraperitoneal and aerosol challenge. All animals vaccinated with the vaccine candidate demonstrated a statistically significant degree of protection against both intraperitoneal and aerosol challenge. Safety was revealed by the absence of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>associated pathological changes, including splenomegaly, hepatomegaly, or granulomatous disease. These results suggest that the 16M\u0394<jats:italic>mucR<\/jats:italic>vaccine is safe, elicits a strong protective immunity, and should be considered as a promising vaccine candidate for human use.<\/jats:p>","DOI":"10.1128\/iai.05330-11","type":"journal-article","created":{"date-parts":[[2011,6,28]],"date-time":"2011-06-28T06:56:41Z","timestamp":1309244201000},"page":"3653-3658","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Protective Efficacy and Safety of Brucella melitensis 16M\u0394<i>mucR<\/i>against Intraperitoneal and Aerosol Challenge in BALB\/c Mice"],"prefix":"10.1128","volume":"79","author":[{"given":"A. 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