{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:12:28Z","timestamp":1761743548841,"version":"3.38.0"},"reference-count":82,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mannheimia haemolytica<\/jats:named-content>is the etiological agent of pneumonic pasteurellosis of cattle and sheep; two different OmpA subclasses, OmpA1 and OmpA2, are associated with bovine and ovine isolates, respectively. These proteins differ at the distal ends of four external loops, are involved in adherence, and are likely to play important roles in host adaptation.<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. haemolytica<\/jats:named-content>is surrounded by a polysaccharide capsule, and the degree of OmpA surface exposure is unknown. To investigate surface exposure and immune specificity of OmpA among bovine and ovine<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. haemolytica<\/jats:named-content>isolates, recombinant proteins representing the transmembrane domain of OmpA from a bovine serotype A1 isolate (rOmpA1) and an ovine serotype A2 isolate (rOmpA2) were overexpressed, purified, and used to generate anti-rOmpA1 and anti-rOmpA2 antibodies, respectively. Immunogold electron microscopy and immunofluorescence techniques demonstrated that OmpA1 and OmpA2 are surface exposed, and are not masked by the polysaccharide capsule, in a selection of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. haemolytica<\/jats:named-content>isolates of various serotypes and grown under different growth conditions. To explore epitope specificity, anti-rOmpA1 and anti-rOmpA2 antibodies were cross-absorbed with the heterologous isolate to remove cross-reacting antibodies. These cross-absorbed antibodies were highly specific and recognized only the OmpA protein of the homologous isolate in Western blot assays. A wider examination of the binding specificities of these antibodies for<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. haemolytica<\/jats:named-content>isolates representing different OmpA subclasses revealed that cross-absorbed anti-rOmpA1 antibodies recognized OmpA1-type proteins but not OmpA2-type proteins; conversely, cross-absorbed anti-rOmpA2 antibodies recognized OmpA2-type proteins but not OmpA1-type proteins. Our results demonstrate that OmpA1 and OmpA2 are surface exposed and could potentially bind to different receptors in cattle and sheep.<\/jats:p>","DOI":"10.1128\/iai.05469-11","type":"journal-article","created":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T02:43:39Z","timestamp":1315363419000},"page":"4332-4341","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Outer Membrane Protein A of Bovine and Ovine Isolates of Mannheimia haemolytica Is Surface Exposed and Contains Host Species-Specific Epitopes"],"prefix":"10.1128","volume":"79","author":[{"given":"Jonathan D. A.","family":"Hounsome","sequence":"first","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Baillie","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojtaba","family":"Noofeli","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Riboldi-Tunnicliffe","sequence":"additional","affiliation":[{"name":"School of Chemistry, College of Science and Engineering, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J. S.","family":"Burchmore","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil W.","family":"Isaacs","sequence":"additional","affiliation":[{"name":"School of Chemistry, College of Science and Engineering, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Davies","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"2415","article-title":"Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica (serotype A1) organisms","volume":"130","author":"Adlam C.","year":"1984","unstructured":"AdlamC.. 1984. Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica (serotype A1) organisms. J. Gen. Microbiol. 130:2415\u20132426.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00297-08"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900557"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02431.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.143.2.906-913.1980"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm047"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4505-4517.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00980-07"},{"key":"e_1_3_2_10_2","first-page":"33","article-title":"Bovine respiratory disease: commercial vaccines currently available in Canada","volume":"41","author":"Bowland S. L.","year":"2000","unstructured":"BowlandS. L.ShewenP. E.. 2000. Bovine respiratory disease: commercial vaccines currently available in Canada. Can. Vet. J. 41:33\u201348.","journal-title":"Can. Vet. J."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.2460\/ajvr.1989.50.04.555","article-title":"Effect of Pasteurella haemolytica (A1) capsular polysaccharide on sheep lung in vivo and on pulmonary surfactant in vitro","volume":"50","author":"Brogden K. A.","year":"1989","unstructured":"BrogdenK. A.. 1989. Effect of Pasteurella haemolytica (A1) capsular polysaccharide on sheep lung in vivo and on pulmonary surfactant in vitro. Am. J. Vet. Res. 50:555\u2013559.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(90)90081-6"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00213-5"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.11.036"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)05390-E"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01239-08"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.16.6.1123-1126.1982"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(89)90016-8"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(03)00098-6"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01786.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.9.3585-3593.1997"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-7-1895"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5741-5752.2004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90072-8"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-5-909"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1394-1404.2001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02838.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03749.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01021.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.8.2998-3006.1996"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)30602-6"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.4.1351-1356.1997"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.554-561.1999"},{"key":"e_1_3_2_34_2","first-page":"197","volume-title":"Pasteurella and pasteurellosis","author":"Frank G. H.","year":"1989","unstructured":"FrankG. H.. 1989. Pasteurellosis of cattle, p. 197\u2013222. In AdlamC.RutterJ. M. (ed.), Pasteurella and pasteurellosis. Academic Press, New York, NY."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(91)90271-7"},{"key":"e_1_3_2_36_2","first-page":"223","volume-title":"Pasteurella and pasteurellosis","author":"Gilmour N. J. L.","year":"1989","unstructured":"GilmourN. J. L.GilmourJ. S.. 1989. Pasteurellosis of sheep, p. 223\u2013262. In AdlamC. RutterJ. M. (ed.), Pasteurella and pasteurellosis. Academic Press, New York, NY."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00675-06"},{"key":"e_1_3_2_38_2","first-page":"D1128","article-title":"Molecular genetic analysis of virulence in Mannheimia (Pasteurella) haemolytica","volume":"6","author":"Highlander S. K","year":"2001","unstructured":"HighlanderS. K. 2001. Molecular genetic analysis of virulence in Mannheimia (Pasteurella) haemolytica. Front. Biosci. 6:D1128\u2013D1151.","journal-title":"Front. Biosci."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02233.x"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0720(15)30903-8"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.06.005"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00312-08"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.8.10.2065"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/35107092"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073413"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01527-09"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2008.03.006"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4458-4464.2001"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00830.x"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/18.1.211"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.1.211-218.1997"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.5.1323-1330.1998"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90586-9"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00047-5"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2622-2629.2002"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.20.5997-6008.2001"},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"875","DOI":"10.2460\/ajvr.1995.56.07.875","article-title":"Vaccination of cattle with outer membrane protein-enriched fractions of Pasteurella haemolytica and resistance against experimental challenge exposure","volume":"56","author":"Morton R. J.","year":"1995","unstructured":"MortonR. J.. 1995. Vaccination of cattle with outer membrane protein-enriched fractions of Pasteurella haemolytica and resistance against experimental challenge exposure. Am. J. Vet. Res. 56:875\u2013879.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.3.711-716.1989"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a131414"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(98)00145-X"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5613-5619.1998"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00293-4"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/2983"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.11.009"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4556-4563.2002"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.1.146-153.1996"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00504a006"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.4.1477-1479.1996"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(74)81210-X"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.12.013"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.5.1249-1257.2004"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00440-08"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00778.x"},{"key":"e_1_3_2_74_2","first-page":"269","article-title":"The susceptibility of in vivo-grown Pasteurella haemolytica to ovine defence mechanisms in vitro","volume":"2","author":"Sutherland A. D.","year":"1990","unstructured":"SutherlandA. D.JonesG. E.PoxtonI. R.. 1990. The susceptibility of in vivo-grown Pasteurella haemolytica to ovine defence mechanisms in vitro. FEMS Microbiol. Immunol. 2:269\u2013278.","journal-title":"FEMS Microbiol. Immunol."},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.4985-4995.2003"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90396-Q"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_18040741.x"},{"key":"e_1_3_2_78_2","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.2460\/ajvr.1985.46.12.2429","article-title":"Changes in leukocyte populations in pulmonary lavage fluids of calves after inhalation of Pasteurella haemolytica","volume":"46","author":"Walker R. D.","year":"1985","unstructured":"WalkerR. D.HopkinsF. M.SchultzT. W.McCrackenM. D.MooreR. N.. 1985. Changes in leukocyte populations in pulmonary lavage fluids of calves after inhalation of Pasteurella haemolytica. Am. J. Vet. Res. 46:2429\u20132433.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-12-1059"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098589002700302"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20853"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4968-4973.1999"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309107028308"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05469-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05469-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T22:02:40Z","timestamp":1741557760000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.05469-11"}},"subtitle":[],"editor":[{"given":"R. P.","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":82,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1128\/IAI.05469-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.05469-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"type":"print","value":"0019-9567"},{"type":"electronic","value":"1098-5522"}],"subject":[],"published":{"date-parts":[[2011,11]]},"assertion":[{"value":"2011-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-08-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-10-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}