{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:25:30Z","timestamp":1791109530869,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The duodenal ulcer promoting (\n            <jats:italic>dupA<\/jats:italic>\n            ) gene, located in the plasticity region of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Helicobacter pylori<\/jats:named-content>\n            , is associated with duodenal ulcer development.\n            <jats:italic>dupA<\/jats:italic>\n            was predicted to form a type IV secretory system (T4SS) with\n            <jats:italic>vir<\/jats:italic>\n            genes around\n            <jats:italic>dupA<\/jats:italic>\n            (\n            <jats:italic>dupA<\/jats:italic>\n            cluster). We investigated the prevalence of\n            <jats:italic>dupA<\/jats:italic>\n            and\n            <jats:italic>dupA<\/jats:italic>\n            clusters and clarified associations between the\n            <jats:italic>dupA<\/jats:italic>\n            cluster status and clinical outcomes in the U.S. population. In all, 245\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            strains were examined using PCR to evaluate the status of\n            <jats:italic>dupA<\/jats:italic>\n            and the adjacent\n            <jats:italic>vir<\/jats:italic>\n            genes predicted to form T4SS, in addition to the status of\n            <jats:italic>cag<\/jats:italic>\n            pathogenicity island (PAI). The associations between\n            <jats:italic>dupA<\/jats:italic>\n            cluster status and interleukin-8 (IL-8) and IL-12 production were also examined. The presence of\n            <jats:italic>dupA<\/jats:italic>\n            and all adjacent\n            <jats:italic>vir<\/jats:italic>\n            genes were defined as a complete\n            <jats:italic>dupA<\/jats:italic>\n            cluster. Many variations related to the status of\n            <jats:italic>dupA<\/jats:italic>\n            and\n            <jats:italic>dupA<\/jats:italic>\n            cluster genes were identified. Concurrent\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            infection and the presence of a complete\n            <jats:italic>dupA<\/jats:italic>\n            cluster increases duodenal ulcer risk compared to\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">H. pylori<\/jats:named-content>\n            infection with incomplete\n            <jats:italic>dupA<\/jats:italic>\n            cluster or without the\n            <jats:italic>dupA<\/jats:italic>\n            gene independent on the\n            <jats:italic>cag<\/jats:italic>\n            PAI status (adjusted odds ratio, 2.13; 95% confidence interval, 1.13 to 4.03). Gastric mucosal IL-8 levels were also significantly higher in the complete\n            <jats:italic>dupA<\/jats:italic>\n            cluster group than in other groups (\n            <jats:italic>P<\/jats:italic>\n            = 0.01). In conclusion, although the causal relationship between the\n            <jats:italic>dupA<\/jats:italic>\n            cluster and duodenal ulcer development is not proved, the presence of a complete\n            <jats:italic>dupA<\/jats:italic>\n            cluster but not\n            <jats:italic>dupA<\/jats:italic>\n            alone, is associated with duodenal ulcer development.\n          <\/jats:p>","DOI":"10.1128\/iai.05472-11","type":"journal-article","created":{"date-parts":[[2011,10,29]],"date-time":"2011-10-29T03:05:58Z","timestamp":1319857558000},"page":"381-387","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["The Intact\n            <i>dupA<\/i>\n            Cluster Is a More Reliable Helicobacter pylori Virulence Marker than\n            <i>dupA<\/i>\n            Alone"],"prefix":"10.1128","volume":"80","author":[{"given":"Sung Woo","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA"},{"name":"Division of Gastroenterology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsushige","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA"},{"name":"Center for Clinical Research, Hamamatsu University School of Medicine, Hamamatsu, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Shiota","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Y.","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Yamaoka","sequence":"additional","affiliation":[{"name":"Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA"},{"name":"Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i43.6815"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2007.00557.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/522177"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01086.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02302.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47776-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(96)90135-5"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00199"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61159-9"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.05.006"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.05977.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/653587"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0933-z"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01737-07"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.1.181-186.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00293-09"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.882-893.2006"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5302-5308.2004"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006859"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.896801"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.01.009"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.011684-0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2008.05762.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0549-8"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.08.019"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-1-5"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-2-13"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra020542"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-009-0772-y"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00286.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa001999"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5493.979"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2010.154"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.4265"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.2008\/000570-0"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.34781"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2258-2263.1998"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.1998.08056.x"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.130079797"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-218"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-8-49"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05472-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05472-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:05:17Z","timestamp":1646481917000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.05472-11"}},"subtitle":[],"editor":[{"given":"S. R.","family":"Blanke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1128\/IAI.05472-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.05472-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1]]},"assertion":[{"value":"2011-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}