{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T01:50:56Z","timestamp":1769824256539,"version":"3.49.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To cause disease,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium requires two type III secretion systems that are encoded by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella<\/jats:named-content>\n            pathogenicity islands 1 and 2 (SPI-1 and -2). These secretion systems serve to deliver specialized proteins (effectors) into the host cell cytosol. While the importance of these effectors to promote colonization and replication within the host has been established, the specific roles of individual secreted effectors in the disease process are not well understood. In this study, we used an\n            <jats:italic>in vivo<\/jats:italic>\n            gallbladder epithelial cell infection model to study the function of the SPI-2-encoded type III effector, SseL. The deletion of the\n            <jats:italic>sseL<\/jats:italic>\n            gene resulted in bacterial filamentation and elongation and the unusual localization of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella<\/jats:named-content>\n            within infected epithelial cells. Infection with the \u0394\n            <jats:italic>sseL<\/jats:italic>\n            strain also caused dramatic changes in host cell lipid metabolism and led to the massive accumulation of lipid droplets in infected cells. This phenotype was directly attributable to the deubiquitinase activity of SseL, as a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella<\/jats:named-content>\n            strain carrying a single point mutation in the catalytic cysteine also resulted in extensive lipid droplet accumulation. The excessive buildup of lipids due to the absence of a functional\n            <jats:italic>sseL<\/jats:italic>\n            gene also was observed in murine livers during\n            <jats:italic>S.<\/jats:italic>\n            Typhimurium infection. These results suggest that SseL alters host lipid metabolism in infected epithelial cells by modifying the ubiquitination patterns of cellular targets.\n          <\/jats:p>","DOI":"10.1128\/iai.05478-11","type":"journal-article","created":{"date-parts":[[2011,8,30]],"date-time":"2011-08-30T00:56:23Z","timestamp":1314665783000},"page":"4392-4400","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["The Deubiquitinase Activity of the Salmonella Pathogenicity Island 2 Effector, SseL, Prevents Accumulation of Cellular Lipid Droplets"],"prefix":"10.1128","volume":"79","author":[{"given":"Ellen T.","family":"Arena","sequence":"first","affiliation":[{"name":"Michael Smith Laboratories, The University of British Columbia, 301-2185 East Mall, Vancouver, British Columbia, Canada V6T 1Z4"},{"name":"Department of Microbiology and Immunology, The University of British Columbia, 301-2185 East Mall, Vancouver, British Columbia, Canada V6T 1Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigrid D.","family":"Auweter","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, The University of British Columbia, 301-2185 East Mall, Vancouver, British Columbia, Canada V6T 1Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Caetano M.","family":"Antunes","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, The University of British Columbia, 301-2185 East Mall, Vancouver, British Columbia, Canada V6T 1Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Wayne","family":"Vogl","sequence":"additional","affiliation":[{"name":"Department of Cellular and Physiological Sciences, Division of Anatomy and Cell Biology, The University of British Columbia, Life Sciences Centre, 3.401-2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Han","sequence":"additional","affiliation":[{"name":"University of Victoria-Genome BC Proteomics Centre, University of Victoria, Victoria, Canada V8Z 7X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian A.","family":"Guttman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Simon Fraser University, Shrum Science Centre, Room B8276, 8888 University Drive, Burnaby, British Columbia, Canada V5A 1S6"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew A.","family":"Croxen","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, The University of British Columbia, 301-2185 East Mall, Vancouver, British Columbia, Canada V6T 1Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Menendez","sequence":"additional","affiliation":[{"name":"Michael Smith Laboratories, The University of British Columbia, 301-2185 East Mall, Vancouver, British Columbia, Canada V6T 1Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott D.","family":"Covey","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The University of British Columbia, Copp Building, Room 1114, Vancouver, British Columbia, Canada V6T 1Z4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph H.","family":"Borchers","sequence":"additional","affiliation":[{"name":"University of Victoria-Genome BC Proteomics Centre, University of Victoria, Victoria, Canada V8Z 7X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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