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While\n Salmonella msbB<\/jats:named-content>\n mutants lacking the myristate chain in lipid A were investigated widely as an anticancer vaccine, inclusion of the\n msbB<\/jats:named-content>\n mutation in a\n Salmonella<\/jats:named-content>\n vaccine to deliver heterologous antigens has not yet been investigated. We introduced the\n msbB<\/jats:named-content>\n mutation alone or in combination with mutations in other lipid A acyl chain modification genes encoding PagL, PagP, and LpxR into wild-type\n S. enterica<\/jats:named-content>\n serovar Typhimurium. The\n msbB<\/jats:named-content>\n mutation reduced virulence, while the\n pagL<\/jats:named-content>\n ,\n pagP<\/jats:named-content>\n , and\n lpxR<\/jats:named-content>\n mutations did not affect virulence in the\n msbB<\/jats:named-content>\n mutant background when administered orally to BALB\/c mice. Also, all mutants exhibited sensitivity to polymyxin B but did not display sensitivity to deoxycholate. LPS derived from\n msbB<\/jats:named-content>\n mutants induced less inflammatory responses in human Mono Mac 6 and murine macrophage RAW264.7 cells\n in vitro<\/jats:italic>\n . However, an\n msbB<\/jats:named-content>\n mutant did not decrease the induction of inflammatory responses in mice compared to the levels induced by the wild-type strain, whereas an\n msbB pagP<\/jats:named-content>\n mutant induced less inflammatory responses\n in vivo<\/jats:italic>\n . The mutations were moved to an attenuated\n Salmonella<\/jats:named-content>\n vaccine strain to evaluate their effects on immunogenicity. Lipid A modification caused by the\n msbB<\/jats:named-content>\n mutation alone and in combination with\n pagL<\/jats:named-content>\n ,\n pagP<\/jats:named-content>\n , and\n lpxR<\/jats:named-content>\n mutations led to higher IgA production in the vaginal tract but still retained the same IgG titer level in serum to PspA, a test antigen from\n Streptococcus pneumoniae<\/jats:named-content>\n , and to outer membrane proteins (OMPs) from\n Salmonella<\/jats:named-content>\n .\n <\/jats:p>","DOI":"10.1128\/iai.05524-11","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T08:11:46Z","timestamp":1316506306000},"page":"5027-5038","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Palmitoylation State Impacts Induction of Innate and Acquired Immunity by the Salmonella enterica Serovar Typhimurium\n msbB<\/i>\n Mutant"],"prefix":"10.1128","volume":"79","author":[{"given":"Qingke","family":"Kong","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology, Biodesign Institute, Arizona State University, Tempe, Arizona 85287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Six","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology, Biodesign Institute, Arizona State University, Tempe, Arizona 85287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth L.","family":"Roland","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology, Biodesign Institute, Arizona State University, Tempe, Arizona 85287"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian R. 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