{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:47:49Z","timestamp":1790776069729,"version":"4.1.0"},"reference-count":83,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Tuberculosis remains a global health threat, and there is dire need to develop a vaccine that is safe and efficacious and confers long-lasting protection. In this study, we constructed recombinant attenuated<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella<\/jats:named-content>vaccine (RASV) strains with plasmids expressing fusion proteins consisting of the 80 amino-terminal amino acids of the type 3 secretion system effector SopE of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella<\/jats:named-content>and the<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>antigens early secreted antigenic target 6-kDa (ESAT-6) protein and culture filtrate protein 10 (CFP-10). We demonstrated that the SopE-mycobacterial antigen fusion proteins were translocated into the cytoplasm of INT-407 cells in cell culture assays. Oral immunization of mice with RASV strains synthesizing SopE\u2013ESAT-6\u2013CFP-10 fusion proteins resulted in significant protection of the mice against aerosol challenge with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">M. tuberculosis<\/jats:named-content>H37Rv that was similar to the protection afforded by immunization with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium bovis<\/jats:named-content>bacillus Calmette-Gu\u00e9rin (BCG) administered subcutaneously. In addition, oral immunization with the RASV strains specifying these mycobacterial antigens elicited production of significant antibody titers to ESAT-6 and production of ESAT-6- or CFP-10-specific gamma interferon (IFN-\u03b3)-secreting and tumor necrosis factor alpha (TNF-\u03b1)-secreting splenocytes.<\/jats:p>","DOI":"10.1128\/iai.05525-11","type":"journal-article","created":{"date-parts":[[2011,12,6]],"date-time":"2011-12-06T06:01:54Z","timestamp":1323151314000},"page":"798-814","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Live Attenuated Salmonella Vaccines against Mycobacterium tuberculosis with Antigen Delivery via the Type III Secretion System"],"prefix":"10.1128","volume":"80","author":[{"given":"Mar\u00eda Dolores","family":"Ju\u00e1rez-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology at Biodesign Institute, Arizona State University, Tempe, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes T.","family":"Arteaga-Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology at Biodesign Institute, Arizona State University, Tempe, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebin","family":"Kader","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology at Biodesign Institute, Arizona State University, Tempe, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Roy","family":"Curtiss","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology at Biodesign Institute, Arizona State University, Tempe, Arizona, USA"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine E.","family":"Clark-Curtiss","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Vaccinology at Biodesign Institute, Arizona State University, Tempe, Arizona, USA"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01959.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.08.044"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5419.1520"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.62.3.293-300.1951"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-11-3195"},{"key":"e_1_3_3_7_2","first-page":"234","article-title":"Curing bacterial cells of lysogenic viruses by using UCB indicator plates","volume":"2","author":"Bochner BR","year":"1984","unstructured":"BochnerBR. 1984. Curing bacterial cells of lysogenic viruses by using UCB indicator plates. Biotechniques 2:234\u2013240.","journal-title":"Biotechniques"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00244-08"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.791-795.2000"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.3.328"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-009-0062-5"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.08.006"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00375-06"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90092-6"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3781740.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2243"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.60.3.992"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.89.1.28-40.1965"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0216941"},{"key":"e_1_3_3_21_2","article-title":"Regulation of attenuation of live vaccines to enhance cross protective immunity.","author":"Curtiss R","year":"2003","unstructured":"CurtissRIII. April 2003. Regulation of attenuation of live vaccines to enhance cross protective immunity. International patent WO 03\/096812.","journal-title":"International patent WO 03\/096812"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.12.3035-3043.1987"},{"key":"e_1_3_3_23_2","article-title":"Regulated bacterial lysis for gene vaccine vector delivery and antigen release.","author":"Curtiss R","year":"2006","unstructured":"CurtissRIIIKongW. June 2006. Regulated bacterial lysis for gene vaccine vector delivery and antigen release. US patent 2006\/0140975 A1.","journal-title":"US patent 2006\/0140975 A1"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00693-08"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v30.i3.30"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03343.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00619-7"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.4.2400-2409.2003"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3242-3247.1999"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)92348-9"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2249"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.9.2596-2602.1994"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.6.1879-1885.1990"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.13.4338-4349.1992"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1322"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.4.6.663-673.1971"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.9.5269"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4121-4130.1995"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.007"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81442-7"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2574"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01441.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02786327"},{"key":"e_1_3_3_44_2","volume-title":"Abstr. 107th Gen. Meet. Am. Soc. Microbiol.","author":"Ju\u00e1rez-Rodr\u00edguez MD","year":"2007","unstructured":"Ju\u00e1rez-Rodr\u00edguezMDClark-CurtissJTorres-EscobarACurtissRIII. 2007. Development of recombinant attenuated Salmonella vaccine (RASV) to deliver Mycobacterium tuberculosis ESAT-6-CFP10 antigens by the type III secretion system, abstr D-091, p 113. Abstr. 107th Gen. Meet. Am. Soc. Microbiol. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.1.307-312.2002"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1739-1749.2002"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.5.1559-1567.2005"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803801105"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00897-08"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00849-3"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.24.7279-7284.2003"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(55)90016-7"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811697106"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2009.58"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.5.1274-1282.1996"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)87499-3"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.158.3.1041-1047.1984"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.3.271"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00109-8"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9833"},{"key":"e_1_3_3_61_2","first-page":"693","article-title":"Construction of an ASD+ expression-cloning vector: stable maintenance and high level expression of cloned genes in a Salmonella vaccine strain","volume":"6","author":"Nakayama K","year":"1988","unstructured":"NakayamaKKellySMCurtissRIII. 1988. Construction of an ASD+ expression-cloning vector: stable maintenance and high level expression of cloned genes in a Salmonella vaccine strain. Biotechnology (NY) 6:693\u2013697.","journal-title":"Biotechnology (NY)"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.078"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.072"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000428"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03531.x"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5376.565"},{"key":"e_1_3_3_67_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"2001","unstructured":"SambrookJRussellDW. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","edition":"3"},{"key":"e_1_3_3_68_2","doi-asserted-by":"crossref","first-page":"238","DOI":"10.4049\/jimmunol.162.1.238","article-title":"IL-4 enhances IL-10 gene expression in murine Th2 cells in the absence of TCR engagement","volume":"162","author":"Schmidt-Weber CB","year":"1999","unstructured":"Schmidt-WeberCBAlexanderSIHenaultLEJamesLLichtmanAH. 1999. IL-4 enhances IL-10 gene expression in murine Th2 cells in the absence of TCR engagement. J. Immunol. 162:238\u2013244.","journal-title":"J. Immunol."},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00363-2"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.214-220.2000"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022172400044685"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80324-6"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.5.1710-1717.1995"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.8.4204"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00005-10"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.06.070"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68507-3"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/44593"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.08.096"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00444-10"},{"key":"e_1_3_3_81_2","volume-title":"Document WHO\/HTM\/TB\/2010.104","author":"World Health Organization","year":"2010","unstructured":"World Health Organization. 2010. Global tuberculosis control surveillance, planning, financing: WHO report 2010. Document WHO\/HTM\/TB\/2010.104. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01623-07"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00457-07"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05525-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05525-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,11]],"date-time":"2023-06-11T13:15:41Z","timestamp":1686489341000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.05525-11"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":83,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1128\/IAI.05525-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.05525-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"2011-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}