{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:28:05Z","timestamp":1791073685852,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Current efforts to develop plague vaccines focus on LcrV, a polypeptide that resides at the tip of type III secretion needles. LcrV-specific antibodies block\n Yersinia pestis<\/jats:named-content>\n type III injection of Yop effectors into host immune cells, thereby enabling phagocytes to kill the invading pathogen. Earlier work reported that antibodies against\n Y. pestis<\/jats:named-content>\n LcrV cannot block type III injection by\n Yersinia enterocolitica<\/jats:named-content>\n strains and suggested that\n lcrV<\/jats:italic>\n polymorphisms may provide for escape from LcrV-mediated plague immunity. We show here that polyclonal or monoclonal antibodies raised against\n Y. pestis<\/jats:named-content>\n KIM D27 LcrV (LcrV\n D27<\/jats:sub>\n ) bind LcrV from\n Y. enterocolitica<\/jats:named-content>\n O:9 strain W22703 (LcrV\n W22703<\/jats:sub>\n ) or O:8 strain WA-314 (LcrV\n WA-314<\/jats:sub>\n ) but are otherwise unable to block type III injection by\n Y. enterocolitica<\/jats:named-content>\n strains. Replacing the\n lcrV<\/jats:italic>\n gene on the pCD1 virulence plasmid of\n Y. pestis<\/jats:named-content>\n KIM D27 with either\n \n lcrV\n W22703<\/jats:sub>\n <\/jats:italic>\n or\n \n lcrV\n WA-314<\/jats:sub>\n <\/jats:italic>\n does not affect the ability of plague bacteria to secrete proteins via the type III pathway, to inject Yops into macrophages, or to cause lethal plague infections in mice. LcrV\n D27<\/jats:sub>\n -specific antibodies blocked type III injection by\n Y. pestis<\/jats:named-content>\n expressing\n \n lcrV\n W22703<\/jats:sub>\n <\/jats:italic>\n or\n \n lcrV\n WA-314<\/jats:sub>\n <\/jats:italic>\n and protected mice against intravenous lethal plague challenge with these strains. Thus, although antibodies raised against LcrV\n D27<\/jats:sub>\n are unable to block the type III injection of\n Y. enterocolitica<\/jats:named-content>\n strains, expression of\n \n lcrV\n W22703<\/jats:sub>\n <\/jats:italic>\n or\n \n lcrV\n WA-314<\/jats:sub>\n <\/jats:italic>\n in\n Y. pestis<\/jats:named-content>\n did not allow these strains to escape LcrV-mediated plague protective immunity in the intravenous challenge model.\n <\/jats:p>","DOI":"10.1128\/iai.05637-11","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T05:17:41Z","timestamp":1326863861000},"page":"1572-1582","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Polymorphisms in the\n lcrV<\/i>\n Gene of Yersinia enterocolitica and Their Effect on Plague Protective Immunity"],"prefix":"10.1128","volume":"80","author":[{"given":"Nathan C.","family":"Miller","sequence":"first","affiliation":[{"name":"Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA, and Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauriane E.","family":"Quenee","sequence":"additional","affiliation":[{"name":"Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA, and Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Elli","sequence":"additional","affiliation":[{"name":"Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA, and Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy A.","family":"Ciletti","sequence":"additional","affiliation":[{"name":"Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA, and Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Schneewind","sequence":"additional","affiliation":[{"name":"Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA, and Department of Microbiology, University of Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.071168"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.6.2590-2599.1994"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-64-15726"},{"key":"e_1_3_2_5_2","first-page":"131","article-title":"Studies on immunization against plague. 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The effect of loss of different virulence determinants on the virulence and immunogenicity of strains of Pasteurella pestis. Br. J. Exp. Pathol. 39:278\u2013291.","journal-title":"Br. J. Exp. Pathol."},{"key":"e_1_3_2_14_2","first-page":"1748","volume-title":"Infectious diseases","author":"Butler T","year":"1995","unstructured":"ButlerT. 1995. Yersinia species, p 1748\u20131756. In MandellGLDouglasRGBennettJE (ed), Infectious diseases, 5th ed. 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