{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:02:51Z","timestamp":1791842571828,"version":"4.3.4"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We report here the identification and characterization of two zinc uptake systems, ZurAM and ZinABC, in the intracellular pathogen\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Listeria monocytogenes<\/jats:named-content>\n            . Transcription of both operons was zinc responsive and regulated by the zinc-sensing repressor Zur. Deletion of either\n            <jats:italic>zurAM<\/jats:italic>\n            or\n            <jats:italic>zinA<\/jats:italic>\n            had no detectable effect on growth in defined media, but a double\n            <jats:italic>zurAM zinA<\/jats:italic>\n            mutant was unable to grow in the absence of zinc supplementation. Deletion of\n            <jats:italic>zinA<\/jats:italic>\n            had no detectable effect on intracellular growth in HeLa epithelial cells. In contrast, growth of the\n            <jats:italic>zurAM<\/jats:italic>\n            mutant was significantly impaired in these cells, indicating the importance of the ZurAM system during intracellular growth. Notably, the deletion of both\n            <jats:italic>zinA<\/jats:italic>\n            and\n            <jats:italic>zurAM<\/jats:italic>\n            severely attenuated intracellular growth, with the double mutant being defective in actin-based motility and unable to spread from cell to cell. Deletion of either\n            <jats:italic>zurAM<\/jats:italic>\n            or\n            <jats:italic>zinA<\/jats:italic>\n            had a significant effect on virulence in an oral mouse model, indicating that both zinc uptake systems are important\n            <jats:italic>in vivo<\/jats:italic>\n            and establishing the importance of zinc acquisition during infection by\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. monocytogenes<\/jats:named-content>\n            . The presence of two zinc uptake systems may offer a mechanism by which\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. monocytogenes<\/jats:named-content>\n            can respond to zinc deficiency within a variety of environments and during different stages of infection, with each system making distinct contributions under different stress conditions.\n          <\/jats:p>","DOI":"10.1128\/iai.05904-11","type":"journal-article","created":{"date-parts":[[2011,10,25]],"date-time":"2011-10-25T02:21:28Z","timestamp":1319509288000},"page":"14-21","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Two Zinc Uptake Systems Contribute to the Full Virulence of Listeria monocytogenes during Growth\n            <i>In Vitro<\/i>\n            and\n            <i>In Vivo<\/i>"],"prefix":"10.1128","volume":"80","author":[{"given":"David","family":"Corbett","sequence":"first","affiliation":[{"name":"Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Wang","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Schuler","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Lopez-Castejon","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, University of Manchester, Manchester, United 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