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In this study, we further identified the receptor of MIMP on NCM460 cells and investigated the mechanism (the p38 mitogen-activated protein kinase [MAPK] pathway) following the interaction of MIMP and its corresponding receptor, mannose receptor. We first identified the target receptor of MIMP on the surfaces of NCM460 cells using immunoprecipitation-mass spectrometry technology. We also verified the mannose receptor and examined the degradation and activation of the p38 MAPK signaling pathway. The results indicated that MIMP adhered to NCM460 cells by binding to the mannose receptor and inhibited the phosphorylation of p38 MAPK stimulated after EPEC infection via inhibition of the Toll-like receptor 5 pathway. These findings indicated that MIMPs relieve the injury of NCM460 cells after enteropathogenic<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>infection through the mannose receptor and inhibition of the p38 MAPK signaling pathway, both of which may therefore be potential therapeutic targets for intestinal diseases, such as inflammatory bowel disease.<\/jats:p>","DOI":"10.1128\/iai.05930-11","type":"journal-article","created":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T07:05:02Z","timestamp":1327993502000},"page":"1343-1350","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Involvement of the Mannose Receptor and p38 Mitogen-Activated Protein Kinase Signaling Pathway of the Microdomain of the Integral Membrane Protein after Enteropathogenic Escherichia coli Infection"],"prefix":"10.1128","volume":"80","author":[{"given":"Zhihua","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China"},{"name":"The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China"},{"name":"Guangdong Gastrointestinal Hospital, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanlei","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Pat","family":"Moyer","sequence":"additional","affiliation":[{"name":"Incell Corporation, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenzhang","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanlong","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01249-09"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000045576.55937.67"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-165"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00141.2003"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.35.4.483"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8344-8351.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.4049\/jimmunol.161.4.1921","article-title":"Role of stress-activated mitogen-activated protein kinase (p38) in beta 2-integrin-dependent neutrophil adhesion and the adhesion-dependent oxidative burst","volume":"161","author":"Detmers PA","year":"1998","unstructured":"DetmersPA. 1998. 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