{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:36:22Z","timestamp":1792730182859,"version":"4.5.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Listeria monocytogenes<\/jats:named-content>\n                    causes a serious food-borne disease due to its ability to spread from the intestine to other organs, a process that is poorly understood. In this study we used 20 signature-tagged wild-type clones of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. monocytogenes<\/jats:named-content>\n                    in guinea pigs in combination with extensive quantitative data analysis to gain insight into extraintestinal dissemination. We show that\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. monocytogenes<\/jats:named-content>\n                    colonized the liver in all asymptomatic animals. Spread to the liver occurred as early as 4 h after ingestion via a direct pathway from the intestine to the liver. This direct pathway contributed significantly to the bacterial load in the liver and was followed by a second wave of dissemination via the mesenteric lymph nodes (indirect pathway). Furthermore, bacteria were eliminated in the liver, whereas small intestinal villi provided a niche for bacterial replication, indicating organ-specific differences in net bacterial growth. Bacteria were shed back from intestinal villi into the small intestinal lumen and reinfected the Peyer's patches. Together, these results support a novel dissemination model where\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. monocytogenes<\/jats:named-content>\n                    replicates in intestinal villi, is shed into the lumen, and reinfects intestinal immune cells that traffic to liver and mesenteric lymph nodes, a process that occurs even during asymptomatic colonization.\n                  <\/jats:p>","DOI":"10.1128\/iai.05958-11","type":"journal-article","created":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T09:16:48Z","timestamp":1321348608000},"page":"720-732","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Oral Infection with Signature-Tagged Listeria monocytogenes Reveals Organ-Specific Growth and Dissemination Routes in Guinea Pigs"],"prefix":"10.1128","volume":"80","author":[{"given":"Jody A.","family":"Melton-Witt","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne M.","family":"Rafelski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, California, USA"},{"name":"School of Public Health, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna I.","family":"Bakardjiev","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Microbial Host and Pathogenesis Program, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"418","article-title":"Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food: 10 states, 2009","volume":"59","year":"2010","unstructured":"Anonymous. 2010. Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food: 10 states, 2009. MMWR Morb. Mortal. Wkly. Rep. 59:418\u2013422.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/430090"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020066"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060905"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/591131"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","article-title":"Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements","volume":"139","author":"Bishop DK","year":"1987","unstructured":"BishopDKHinrichsDJ. 1987. Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements. J. Immunol. 139:2005\u20132009.","journal-title":"J. Immunol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2460\/ajvr.1992.53.02.191","article-title":"Biliary excretion as possible origin of Listeria monocytogenes in fecal carriers","volume":"53","author":"Briones V","year":"1992","unstructured":"BrionesV. 1992. Biliary excretion as possible origin of Listeria monocytogenes in fecal carriers. Am. J. Vet. Res. 53:191\u2013193.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_9_2","first-page":"Unit 19","article-title":"Animal model for infection with Listeria monocytogenes","volume":"19","author":"Busch DH","year":"2001","unstructured":"BuschDHVijhSPamerEG. 2001. Animal model for infection with Listeria monocytogenes. Curr. Protoc. Immunol. Chapter 19:Unit 19.","journal-title":"Curr. Protoc. Immunol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.1237-1243.1998"},{"key":"e_1_3_2_11_2","unstructured":"CooperGSchillerAL. 1975. Anatomy of the guinea pig. Harvard University Press Cambridge MA."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.682-689.2003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091490315"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00404.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1344-1350.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01765.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060074"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.30.2.309-382.1966"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02913723"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.3.673-678.1989"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092712"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.3.435"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/420853"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.8.3758-3766.1998"},{"key":"e_1_3_2_25_2","first-page":"204","article-title":"The gastrointestinal transit time in the guinea-pig","volume":"22","author":"Jilge B","year":"1980","unstructured":"JilgeB. 1980. The gastrointestinal transit time in the guinea-pig. Zentbl. Versuchstierkd. 22:204\u2013210.","journal-title":"Zentbl. Versuchstierkd."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.15"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006098107"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.15.4177-4186.2002"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.009"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.14.3956"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059852"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"980","DOI":"10.4049\/jimmunol.111.3.980","article-title":"Immunologic responses in the gastrointestinal tract of the guinea pig. I. Characterization of Peyer's patch cells","volume":"111","author":"Levin DM","year":"1973","unstructured":"LevinDMRosenstreichDLReynoldsHY. 1973. Immunologic responses in the gastrointestinal tract of the guinea pig. I. Characterization of Peyer's patch cells. J. Immunol. 111:980\u2013983.","journal-title":"J. Immunol."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1159\/000321157"},{"key":"e_1_3_2_34_2","first-page":"1","article-title":"Surveillance for foodborne-disease outbreaks\u2013United States, 1998\u20132002","volume":"55","author":"Lynch M","year":"2006","unstructured":"LynchMPainterJWoodruffRBradenC. 2006. Surveillance for foodborne-disease outbreaks\u2013United States, 1998\u20132002. MMWR Surveill. Summ. 55:1\u201342.","journal-title":"MMWR Surveill. Summ."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(92)90090-H"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2779-2787.2001"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81070-3"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00866.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-61.2.244"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020003"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205009"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00120.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.747-755.1998"},{"key":"e_1_3_2_45_2","first-page":"694","article-title":"Experimental Listeria enteritis. I. An electron microscopic study of the epithelial phase in experimental listeria infection","volume":"26","author":"Racz P","year":"1972","unstructured":"RaczPTennerKMeroE. 1972. Experimental Listeria enteritis. I. An electron microscopic study of the epithelial phase in experimental listeria infection. Lab. Invest. 26:694\u2013700.","journal-title":"Lab. Invest."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.6.1333"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000732"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198301273080407"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/357257a0"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/44593"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00880.x"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.8.6.618"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.049"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05958-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.05958-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,14]],"date-time":"2024-04-14T14:53:51Z","timestamp":1713106431000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.05958-11"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1128\/IAI.05958-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.05958-11","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.13952960.15408062","asserted-by":"object"}]},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]},"assertion":[{"value":"2011-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}