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One effector, YopE, functions as a Rho GTPase-activating protein (GAP). In addition to acting as a virulence factor, YopE can function as a protective antigen. C57BL\/6 mice infected with attenuated<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Yersinia pestis<\/jats:named-content>generate a dominant H2-K<jats:sup>b<\/jats:sup>-restricted CD8 T cell response to an epitope in the N-terminal domain of YopE (YopE<jats:sub>69-77<\/jats:sub>), and intranasal vaccination with the YopE<jats:sub>69-77<\/jats:sub>peptide and the mucosal adjuvant cholera toxin (CT) elicits CD8 T cells that are protective against lethal pulmonary challenge with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pestis<\/jats:named-content>. Because YopE<jats:sub>69-77<\/jats:sub>is conserved in many<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Yersinia<\/jats:named-content>strains, we sought to determine if YopE is a protective antigen for<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Yersinia pseudotuberculosis<\/jats:named-content>and if primary infection with this enteric pathogen elicits a CD8 T cell response to this epitope. Intranasal immunization with the YopE<jats:sub>69-77<\/jats:sub>peptide and CT elicited a CD8 T cell response that was protective against lethal intragastric<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pseudotuberculosis<\/jats:named-content>challenge. The YopE<jats:sub>69-77<\/jats:sub>epitope was a major antigen (\u223c30% of splenic CD8 T cells were specific for this peptide at the peak of the response) during primary infection with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pseudotuberculosis<\/jats:named-content>, as shown by flow cytometry tetramer staining. Results of infections with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pseudotuberculosis<\/jats:named-content>expressing catalytically inactive YopE demonstrated that GAP activity is dispensable for a CD8 T cell response to YopE<jats:sub>69-77<\/jats:sub>. Determining the features of YopE that are important for this response will lead to a better understanding of how protective CD8 T cell immunity is generated against<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Yersinia<\/jats:named-content>and other pathogens with type III secretion systems.<\/jats:p>","DOI":"10.1128\/iai.05971-11","type":"journal-article","created":{"date-parts":[[2011,11,8]],"date-time":"2011-11-08T08:23:31Z","timestamp":1320740611000},"page":"206-214","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["A Protective Epitope in Type III Effector YopE Is a Major CD8 T Cell Antigen during Primary Infection with Yersinia pseudotuberculosis"],"prefix":"10.1128","volume":"80","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio","family":"Mena","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Romanov","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jr-Shiuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Saranac Lake, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen T.","family":"Smiley","sequence":"additional","affiliation":[{"name":"Trudeau Institute, Saranac Lake, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B.","family":"Bliska","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases and Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14043"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2010.12.004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.04.007"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00684.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01110.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00114.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00191545"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.3.1140-1149.1992"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.07.010"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000573"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02021.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00034-08"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708344105"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.12.004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000067"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.04.009"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01250-09"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1526"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1315-1352.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-72124-8_38"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0230-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01360.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.4049\/jimmunol.162.5.2875","article-title":"Coordinate involvement of invasin and Yop proteins in a Yersinia pseudotuberculosis-specific class I-restricted cytotoxic T cell-mediated response","volume":"162","author":"Falgarone G","year":"1999","unstructured":"FalgaroneGBlanchardHSVirecoulonFSimonetMBrebanM. 1999. 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