{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T10:09:52Z","timestamp":1778580592020,"version":"3.51.4"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p><jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>is a significant human respiratory pathogen that causes high morbidity worldwide. No vaccine to prevent<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>infection currently exists, since the mechanisms of pathogenesis are poorly understood. To this end, we constructed a P30 cytadhesin mutant (P-130) with a drastically reduced capacity for binding to erythrocytes and an inability to glide on glass substrates. This mutant was determined to be avirulent and cannot survive in the lungs of BALB\/c mice. We also ascertained that the previously identified P30 gliding motility mutant II-3R is avirulent and also cannot be recovered from the lungs of mice after infection. Mutant P130 was then assessed for its efficacy as a live attenuated vaccine candidate in mice after challenge with wild-type<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>. After vaccination with the P-130 P30 mutant, mice showed evidence of exacerbated disease upon subsequent challenge with the wild-type strain PI1428, which appears to be driven by a Th17 response and corresponding eosinophilia. Our results are in accordance with other reports of vaccine-induced disease exacerbation in rodents and emphasize the need to better understand the basic mechanisms of<jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>pathogenesis.<\/jats:p>","DOI":"10.1128\/iai.06078-11","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T05:17:41Z","timestamp":1326863861000},"page":"1007-1014","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Vaccination of BALB\/c Mice with an Avirulent Mycoplasma pneumoniae P30 Mutant Results in Disease Exacerbation upon Challenge with a Virulent Strain"],"prefix":"10.1128","volume":"80","author":[{"given":"S. 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