{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:02:42Z","timestamp":1791424962344,"version":"4.3.3"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n                    The Reg3 protein family, including the human member designated pancreatitis-associated protein (PAP), consists of secreted proteins that contain a C-type lectin domain involved in carbohydrate binding. They are expressed by intestinal epithelial cells. Colonization of germ-free mice and intestinal infection with pathogens increase the expression of Reg3g and Reg3b in the murine ileum. Reg3g is directly bactericidal for Gram-positive bacteria, but the exact role of Reg3b in bacterial infections is unknown. To investigate the possible protective role of Reg3b in intestinal infection, Reg3b knockout (Reg3b\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice and wild-type (WT) mice were orally infected with Gram-negative\n                    <jats:named-content content-type=\"genus-species\">Salmonella enteritidis<\/jats:named-content>\n                    or Gram-positive\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    . At day 2 after oral\n                    <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n                    infection and at day 4 after oral\n                    <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n                    infection, mice were sacrificed to collect intestinal and other tissues for pathogen quantification. Protein expression of Reg3b and Reg3g was determined in intestinal mucosal scrapings of infected and noninfected mice. In addition,\n                    <jats:italic>ex vivo<\/jats:italic>\n                    binding of ileal mucosal Reg3b to\n                    <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n                    was investigated. Whereas recovery of\n                    <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n                    or\n                    <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n                    from feces of Reg3b\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice did not differ from that from feces of WT mice, significantly higher numbers of viable\n                    <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n                    , but not\n                    <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n                    , bacteria were recovered from the colon, mesenteric lymph nodes, spleen, and liver of the Reg3b\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice than from those of WT mice. Mucosal Reg3b binds to both bacterial pathogens and may interfere with their mode of action. Reg3b plays a protective role against intestinal translocation of the Gram-negative bacterium\n                    <jats:named-content content-type=\"genus-species\">S. enteritidis<\/jats:named-content>\n                    in mice but not against the Gram-positive bacterium\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/iai.06165-11","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T00:17:41Z","timestamp":1326845861000},"page":"1115-1120","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["Intestinally Secreted C-Type Lectin Reg3b Attenuates Salmonellosis but Not Listeriosis in Mice"],"prefix":"10.1128","volume":"80","author":[{"given":"Marleen T. J.","family":"van Ampting","sequence":"first","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Department of Health, NIZO food research, Ede, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M. P.","family":"Loonen","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Host Microbe Interactomics Group, Animal Sciences Department, Wageningen University, Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan J.","family":"Schonewille","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Department of Health, NIZO food research, Ede, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Konings","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Host Microbe Interactomics Group, Animal Sciences Department, Wageningen University, Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolien","family":"Vink","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Department of Health, NIZO food research, Ede, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Iovanna","sequence":"additional","affiliation":[{"name":"INSERM U624, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Chamaillard","sequence":"additional","affiliation":[{"name":"INSERM U801, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Dekker","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roelof","family":"van der Meer","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry M.","family":"Wells","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Host Microbe Interactomics Group, Animal Sciences Department, Wageningen University, Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingeborg M. J.","family":"Bovee-Oudenhoven","sequence":"additional","affiliation":[{"name":"TI Food and Nutrition, Wageningen, The Netherlands"},{"name":"Department of Health, NIZO food research, Ede, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.8.4453"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070563"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127119"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090124"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.168443"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408419509113535"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-78-2-271"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.1.364-367.1993"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1995.1002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0b013e3282f21388"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026088915240"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.056028"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.009"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909449107"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21434"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00992.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808077200"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(94)90059-0"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.012"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.12.1916089"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.11.1939"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00190.2006"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.33.7.987"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00657.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-008-0685-0"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6793-9-6"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.04.001"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(89)90001-9"},{"key":"e_1_3_3_30_2","unstructured":"WHO. 2007. 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L.","family":"Flynn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1128\/IAI.06165-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.06165-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]},"assertion":[{"value":"2011-11-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}