{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:06:08Z","timestamp":1791223568717,"version":"4.1.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            spp. are food- and waterborne pathogens that cause severe diarrheal and dysenteric disease associated with high morbidity and mortality. Individuals most often affected are children under 5 years of age in the developing world. The existence of multiple\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            serotypes and the heterogenic distribution of pathogenic strains, as well as emerging antibiotic resistance, require the development of a broadly protective vaccine. All\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            spp. utilize a type III secretion system (TTSS) to initiate infection. The type III secretion apparatus (TTSA) is the molecular needle and syringe that form the energized conduit between the bacterial cytoplasm and the host cell to transport effector proteins that manipulate cellular processes to benefit the pathogen. IpaB and IpaD form a tip complex atop the TTSA needle and are required for pathogenesis. Because they are common to all virulent\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            spp., they are ideal candidate antigens for a subunit-based, broad-spectrum vaccine. We examined the immunogenicity and protective efficacy of IpaB and IpaD, alone or combined, coadministered with a double mutant heat-labile toxin (dmLT) from\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            , used as a mucosal adjuvant, in a mouse model of intranasal immunization and pulmonary challenge. Robust systemic and mucosal antibody- and T cell-mediated immunities were induced against both proteins, particularly IpaB. Mice immunized in the presence of dmLT with IpaB alone or IpaB combined with IpaD were fully protected against lethal pulmonary infection with\n            <jats:named-content content-type=\"genus-species\">Shigella flexneri<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Shigella sonnei<\/jats:named-content>\n            . We provide the first demonstration that the\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            TTSAs IpaB and IpaD are promising antigens for the development of a cross-protective\n            <jats:named-content content-type=\"genus-species\">Shigella<\/jats:named-content>\n            vaccine.\n          <\/jats:p>","DOI":"10.1128\/iai.06174-11","type":"journal-article","created":{"date-parts":[[2011,12,28]],"date-time":"2011-12-28T02:44:24Z","timestamp":1325040264000},"page":"1222-1231","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":144,"title":["Broadly Protective Shigella Vaccine Based on Type III Secretion Apparatus Proteins"],"prefix":"10.1128","volume":"80","author":[{"given":"Francisco J.","family":"Martinez-Becerra","sequence":"first","affiliation":[{"name":"Center for Vaccine Development and Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian M.","family":"Kissmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jovita","family":"Diaz-McNair","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development and Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyamal P.","family":"Choudhari","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy M.","family":"Quick","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Mellado-Sanchez","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development and Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Clements","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela F.","family":"Pasetti","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development and Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy L.","family":"Picking","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.11.001"},{"key":"e_1_3_3_3_2","article-title":"Identification of the bile salt binding site on IpaD from Shigella flexneri and the influence of ligand binding on IpaD structure","author":"Barta ML","year":"2011","unstructured":"Barta ML , et al.. 23 December 2011, posting date. 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