{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:53:20Z","timestamp":1781830400463,"version":"3.54.5"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Group B streptococci (GBS;\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptococcus agalactiae<\/jats:named-content>\n            ) are the most common cause of neonatal sepsis and meningitis. Serotype-specific IgG antibody is known to protect neonates against GBS infections by promoting opsonophagocytosis. The L-ficolin-mediated lectin pathway of the complement is also a potential mechanism for opsonization of GBS, because L-ficolin activates the complement after binding to serotype Ib, III, V, VI, and VIII GBS. In the present study, we investigated how L-ficolin and serotype-specific IgG in cord sera contribute to opsonophagocytic killing of GBS. Neither L-ficolin nor serotype-specific IgG concentrations correlated with C3b deposition on serotype Ib and VI GBS, suggesting L-ficolin- and serotype-specific IgG-independent mechanisms of complement activation. The percentage of serotype VIII GBS killed was high regardless of the concentration of L-ficolin and IgG. In contrast, L-ficolin and serotype-specific IgG can each initiate C3b deposition on serotype III and V GBS and promote phagocytosis by polymorphonuclear leukocytes, but L-ficolin and serotype-specific IgG together promote opsonophagocytic killing to a greater extent than does either alone\n            <jats:italic>in vitro<\/jats:italic>\n            . This synergy was observed when serotype III-specific IgG concentrations were between 1 and 6 \u03bcg\/ml and when serotype V-specific IgG concentrations were between 2 and 5 \u03bcg\/ml. Concentrations of serotype III-specific IgG in cord blood above 7 \u03bcg\/ml are considered protective for neonates colonized with GBS, but most neonates with IgG levels of less than 7 \u03bcg\/ml do not develop GBS infections. The data presented here suggest that L-ficolin enhances opsonophagocytosis of serotype III and V GBS when serotype-specific IgG alone is suboptimal for protection.\n          <\/jats:p>","DOI":"10.1128\/iai.06232-11","type":"journal-article","created":{"date-parts":[[2012,3,27]],"date-time":"2012-03-27T03:03:08Z","timestamp":1332817388000},"page":"2053-2060","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["L-Ficolin and Capsular Polysaccharide-Specific IgG in Cord Serum Contribute Synergistically to Opsonophagocytic Killing of Serotype III and V Group B Streptococci"],"prefix":"10.1128","volume":"80","author":[{"given":"Mioko","family":"Fujieda","sequence":"first","affiliation":[{"name":"Divisions of Microbiology, Joshi-Eiyoh (Kagawa Nutrition) University, Sakado, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youko","family":"Aoyagi","sequence":"additional","affiliation":[{"name":"Divisions of Microbiology, Joshi-Eiyoh (Kagawa Nutrition) University, Sakado, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousaku","family":"Matsubara","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics, Nishi-Kobe Medical Center, Kobe, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology, Nishi-Kobe Medical Center, Kobe, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wakae","family":"Fujimaki","sequence":"additional","affiliation":[{"name":"Human Medical Science, Joshi-Eiyoh (Kagawa Nutrition) University, Sakado, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misao","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Department of Applied Biochemistry, Tokai University, Hiratsuka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Bohnsack","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Divisions of Microbiology, Joshi-Eiyoh (Kagawa Nutrition) University, Sakado, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00837-07"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.1.418"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00353-0"},{"key":"e_1_3_3_5_2","first-page":"1","article-title":"Synergy, additivism and antagonism in immunosuppression. A critical review","volume":"28","author":"Berenbaum MC","year":"1977","unstructured":"BerenbaumMC. 1977. Synergy, additivism and antagonism in immunosuppression. A critical review. Clin. Exp. Immunol. 28:1\u201318.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02105-07"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.13.4615-4626.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2302-2308.2001"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.5.1275"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.532"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1442-200X.2002.01638.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi173"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01188.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2530-2536.2003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2009.04.003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407161200"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/314666"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2005.090472"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1257-1261.2006"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/323350"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/422756"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01424-08"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.06.020"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/342411"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1159\/000228160"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2001.1800107.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-2985-00149"},{"key":"e_1_3_3_28_2","first-page":"135","volume-title":"Experimental immunochemistry","author":"Mayer MM","year":"1961","unstructured":"MayerMM. 1961. Complement and complement fixation. Hemolytic assay of complement, p 135\u2013139. In KabatEAMayerMM (ed), Experimental immunochemistry, 2nd ed. Charles C. Thomas, Springfield, IL.","edition":"2"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2007.01915.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.12.4731"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-35-5-297"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.4.1415-1420.1993"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1086\/510627"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.497"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.07.025"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1086\/517408"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1086\/342950"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1866-1870.1999"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.12.4764-4769.1995"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.25.11490"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.06232-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.06232-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T20:06:42Z","timestamp":1646424402000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.06232-11"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1128\/IAI.06232-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.06232-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6]]},"assertion":[{"value":"2011-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-05-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}