{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:36:04Z","timestamp":1790980564981,"version":"4.1.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Brucella ovis<\/jats:named-content>\n is a rough bacterium\u2014lacking O-polysaccharide chains in the lipopolysaccharide\u2014that is virulent in its natural host and whose virulence mechanisms remain almost unexplored. In a search for additional traits that distinguish\n B. ovis<\/jats:named-content>\n from smooth\n Brucella<\/jats:named-content>\n , which require O-polysaccharide chains for virulence, we have analyzed the significance in\n B. ovis<\/jats:named-content>\n of the main virulence factors described for smooth\n Brucella<\/jats:named-content>\n . Attempts to obtain strains of virulent\n B. ovis<\/jats:named-content>\n strain PA that are mutated in the BvrR\/BvrS two-component regulatory system were unsuccessful, suggesting the requirement of that system for\n in vitro<\/jats:italic>\n survival, while the inactivation of\n bacA<\/jats:italic>\n \u2014in contrast to the results seen with smooth\n Brucella<\/jats:named-content>\n \u2014did not affect splenic colonization in mice or behavior in J774.A1 murine macrophages. Defects in the synthesis of cyclic \u00df-1,2 glucans reduced the uptake of\n B. ovis<\/jats:named-content>\n PA in macrophages and, although the intracellular multiplication rate was unaffected, led to attenuation in mice. Growth of strains with mutations in the type IV secretion system (encoded by the\n virB<\/jats:italic>\n operon) and the quorum-sensing-related regulator VjbR was severely attenuated in the mouse model, and although the mutant strains internalized like the parental strain in J774.A1 murine macrophages, they were impaired for intracellular replication. As described for\n B. melitensis<\/jats:named-content>\n , VjbR regulates the transcription of the\n virB<\/jats:italic>\n operon positively, and the\n N<\/jats:italic>\n -dodecanoyl-\n dl<\/jats:sc>\n -homoserine lactone (C\n 12<\/jats:sub>\n -HSL) autoinducer abrogates this effect. In contrast, no apparent VjbR-mediated regulation of the\n fliF<\/jats:italic>\n flagellar gene was observed in\n B. ovis<\/jats:named-content>\n , probably due to the two deletions detected upstream of\n fliF<\/jats:italic>\n . These results, together with others reported in the text, point to similarities between rough virulent\n B. ovis<\/jats:named-content>\n and smooth\n Brucella<\/jats:named-content>\n species as regards virulence but also reveal distinctive traits that could be related to the particular pathogenicity and host tropism characteristics of\n B. ovis<\/jats:named-content>\n .\n <\/jats:p>","DOI":"10.1128\/iai.06257-11","type":"journal-article","created":{"date-parts":[[2012,3,6]],"date-time":"2012-03-06T04:33:19Z","timestamp":1331008399000},"page":"1783-1793","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Quorum-Sensing and BvrR\/BvrS Regulation, the Type IV Secretion System, Cyclic Glucans, and BacA in the Virulence of Brucella ovis: Similarities to and Differences from Smooth Brucellae"],"prefix":"10.1128","volume":"80","author":[{"given":"Ana 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