{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T23:04:29Z","timestamp":1791759869713,"version":"4.3.4"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptococcus suis<\/jats:named-content>serotype 2 is a Gram-positive bacterium that causes sepsis and meningitis in piglets and humans. The mechanisms of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. suis<\/jats:named-content>serotype 2 invasive disease are not well understood. The surface proteins of pathogens usually play important roles in infection and bacterium-host interactions. Here, we identified a novel surface protein that contributed significantly to the virulence of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. suis<\/jats:named-content>serotype 2 in a piglet infection model. This protein showed little similarity to other reported proteins and exhibited strong binding activity to human factor H (hFH). It was designated Fhb (factor H-binding protein). The<jats:italic>fhb<\/jats:italic>genes found in<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. suis<\/jats:named-content>serotypes 1, 2, 4, 7, and 9 exhibited molecular polymorphism. Fhb possessed two proline-rich repeat sequences and XPZ domains, and one repeat sequence exhibited a high homology to Bac, an IgA-binding protein of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptococcus agalactiae<\/jats:named-content>. Evidence strongly indicated that<jats:italic>fhb<\/jats:italic>-deficient mutants had diminished phagocytosis resistance in bactericidal assays. In addition, Fhb plays important roles in complement-mediated immunity by interacting with hFH. These findings indicated that Fhb is a crucial surface protein contributing to the virulence of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. suis<\/jats:named-content>, with important functions in evading innate immune defenses by interaction with host complement regulatory factor hFH.<\/jats:p>","DOI":"10.1128\/iai.06294-11","type":"journal-article","created":{"date-parts":[[2012,4,24]],"date-time":"2012-04-24T04:49:54Z","timestamp":1335242994000},"page":"2402-2413","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":91,"title":["Fhb, a Novel Factor H-Binding Surface Protein, Contributes to the Antiphagocytic Ability and Virulence of Streptococcus suis"],"prefix":"10.1128","volume":"80","author":[{"given":"Yaya","family":"Pian","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhen","family":"Gan","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujie","family":"Wang","sequence":"additional","affiliation":[{"name":"Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Hei Long Jiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"},{"name":"CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingping","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehui","family":"Cai","sequence":"additional","affiliation":[{"name":"Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Hei Long Jiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.1.131"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112072200"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.4049\/jimmunol.118.2.673","article-title":"Antigenic specificity of opsonophagocytic antibodies in rabbit anti-sera to group B streptococci","volume":"118","author":"Baltimore RS","year":"1977","unstructured":"BaltimoreRSKasperDLBakerCJGoroffDK. 1977. Antigenic specificity of opsonophagocytic antibodies in rabbit anti-sera to group B streptococci. J. Immunol. 118:673\u2013678.","journal-title":"J. Immunol."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S146625230999003X"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00359-06"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02390-2"},{"key":"e_1_3_3_8_2","first-page":"236","article-title":"Dilemma of virulence of Streptococcus suis: Canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs","volume":"69","author":"Berthelot-Herault F","year":"2005","unstructured":"Berthelot-HeraultFGottschalkMMorvanHKobischM. 2005. Dilemma of virulence of Streptococcus suis: Canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs. Can. J. Vet. Res. 69:236\u2013240.","journal-title":"Can. J. Vet. Res."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00576-0"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.01.007"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04527.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.06067.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2006.04.001"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01944.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(17)30581-X"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04977.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.026765-0"},{"key":"e_1_3_3_18_2","first-page":"66","article-title":"Interaction of human factor H with PspC of Streptococcus pneumoniae","volume":"119","author":"Dave S","year":"2004","unstructured":"DaveSPangburnMKPruittCMcDanielLS. 2004. Interaction of human factor H with PspC of Streptococcus pneumoniae. Indian J. Med. Res. 119(Suppl):66\u201373.","journal-title":"Indian J. Med. Res."},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.471"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1319-1325.2002"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1990485"},{"key":"e_1_3_3_22_2","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.4049\/jimmunol.123.3.1195","article-title":"Studies of the molecular mechanisms of C3b inactivation and a simplified assay of \u03b21H and the C3b inactivator (C3bINA)","volume":"123","author":"Gaither TA","year":"1979","unstructured":"GaitherTAHammerCHFrankMM. 1979. Studies of the molecular mechanisms of C3b inactivation and a simplified assay of \u03b21H and the C3b inactivator (C3bINA). J. Immunol. 123:1195\u20131204.","journal-title":"J. Immunol."},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr800196v"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00421-7"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00250-9"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00011230"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/518509"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210623"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2003.00076.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.4.1886"},{"key":"e_1_3_3_31_2","first-page":"39","article-title":"Streptococcus suis infection in Hong Kong","volume":"88","author":"Kay R","year":"1995","unstructured":"KayRChengAFTseCY. 1995. Streptococcus suis infection in Hong Kong. QJM 88:39\u201347.","journal-title":"QJM"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2004.06.017"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3342-3350.2005"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1201\/b14212-6"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir415"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.676-681.2003"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00046-07"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602404200"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28535-0"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.63.1.174-229.1999"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.184.4.971-982.2002"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-005-0243-8"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.11.6899"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009396200"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004300050028"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01727-06"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-588"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00717-07"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5322-5330.2004"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030187"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00147-9"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005870317757"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.2001.1532"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030151"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2001.00460.x"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(88)90008-9"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90546-N"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00792.x"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2008.10.003"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0337370100"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-008-0425-z"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001463"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.31.18892"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.08.010"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807087200"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.3.1813"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.06.074"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.06294-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.06294-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T06:50:21Z","timestamp":1687416621000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.06294-11"}},"subtitle":[],"editor":[{"given":"R. P.","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":66,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1128\/IAI.06294-11"],"URL":"https:\/\/doi.org\/10.1128\/iai.06294-11","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7]]},"assertion":[{"value":"2011-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}