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The highly complex genome of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            is comprised of a linear chromosome and a large number of variably sized linear and circular plasmids. Many plasmids of this spirochete are unstable during its culture\n            <jats:italic>in vitro<\/jats:italic>\n            . Given that many of the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            virulence factors identified to date are plasmid encoded, spirochetal plasmid content determination is essential for genetic analysis of Lyme pathogenesis. Although PCR-based assays facilitate plasmid profiling of sequenced\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            strains, a rapid genetic content determination strategy for nonsequenced strains has not yet been described. In this study, we combined pulsed-field gel electrophoresis (PFGE) and Southern hybridization for detection of genes encoding known virulence factors,\n            <jats:underline>r<\/jats:underline>\n            ibosomal RNA gene\n            <jats:underline>s<\/jats:underline>\n            pacer restriction fragment length polymorphism\n            <jats:underline>t<\/jats:underline>\n            ypes (RSTs),\n            <jats:italic>ospC<\/jats:italic>\n            group determination, and sequencing of the variable\n            <jats:italic>dbpA<\/jats:italic>\n            and\n            <jats:italic>ospC<\/jats:italic>\n            genes. We show that two strains isolated from the same tick and both originally named N40 are in fact very distinct. Furthermore, we failed to detect\n            <jats:italic>bbk32<\/jats:italic>\n            , which encodes a fibronectin-binding adhesin, in one \u201cN40\u201d strain. Thus, two distinct strains that show different plasmid profiles, as determined by PFGE and PCR, were isolated from the same tick and vary in their\n            <jats:italic>ospC<\/jats:italic>\n            and\n            <jats:italic>dbpA<\/jats:italic>\n            sequences. However, both belong to group RST3B.\n          <\/jats:p>","DOI":"10.1128\/iai.06326-11","type":"journal-article","created":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T07:05:02Z","timestamp":1327993502000},"page":"1519-1529","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Detection of Established Virulence Genes and Plasmids To Differentiate Borrelia burgdorferi Strains"],"prefix":"10.1128","volume":"80","author":[{"given":"Kamfai","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherwood","family":"Casjens","sequence":"additional","affiliation":[{"name":"Division of Microbiology and Immunology, Department of Pathology, University of Utah Medical School, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhat","family":"Parveen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1526-1532.1999"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/314613"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05924.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.02.008"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700024"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.3.475-478.1988"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00153-10"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(89)90083-1"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.2.419"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.133"},{"key":"e_1_3_3_12_2","first-page":"959","article-title":"Chronic Lyme borreliosis in the laboratory mouse","volume":"143","author":"Barthold SW","year":"1993","unstructured":"BartholdSWde SouzaMSJanotkaJLSmithALPersingDH. 1993. 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