{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:44:33Z","timestamp":1791146673481,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Borrelia hermsii<\/jats:named-content>\n and other relapsing fever (RF) species are noted for their highly polymorphic surface antigens, the variable major proteins (VMP). Less is known about other surface proteins of these pathogens in either their vertebrate reservoirs or arthropod vectors. To further characterize these proteins, we elicited antibodies against VMP-less cells, noted antibody reactions against whole cells and cell components, and then subjected selected antigens to mass spectroscopy for amino acid sequencing for comparison against a\n B. hermsii<\/jats:named-content>\n genome database. One of the derived monoclonal antibodies, H0120, agglutinated spirochetes, and in Western blot analyses, it bound to a 14-kDa protein of whole cells and their membrane fractions but not after protease treatment. A search of open reading frames of the\n B. hermsii<\/jats:named-content>\n genome with extracted peptides identified the 14-kDa protein with\n bha128<\/jats:italic>\n , a 453-nucleotide gene of the 175-kb linear plasmid. The\n bha128<\/jats:italic>\n gene was synthesized and expressed in\n Escherichia coli<\/jats:named-content>\n . The protein product was bound by antibody H0120. Genes homologous to\n bha128<\/jats:italic>\n occur in the RF species\n Borrelia turicatae<\/jats:named-content>\n ,\n B. duttonii<\/jats:named-content>\n , and\n B. recurrentis<\/jats:named-content>\n but not in Lyme disease\n Borrelia<\/jats:named-content>\n species or other organisms. The following findings indicated an association of BHA128, renamed Alp, with the tick environment: (i) Alp was produced at higher levels at 23\u00b0C than at 34\u00b0C; (ii) almost all spirochetes in tick salivary glands were bound by the H0120 antibody, but only \u223c1% of spirochetes in the blood of infected mice were bound; and (iii) infected mice produced antibodies to several\n B. hermsii<\/jats:named-content>\n antigens but not detectably to native or recombinant Alp.\n <\/jats:p>","DOI":"10.1128\/iai.06419-11","type":"journal-article","created":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T02:35:46Z","timestamp":1329878146000},"page":"1881-1890","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Alp, an Arthropod-Associated Outer Membrane Protein of Borrelia Species That Cause Relapsing Fever"],"prefix":"10.1128","volume":"80","author":[{"given":"Renee A.","family":"Marcsisin","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics, Medicine, and Ecology and Evolutionary Biology, University of California, Irvine, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley A.","family":"Campeau","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics, Medicine, and Ecology and Evolutionary Biology, University of California, Irvine, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Job E.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan G.","family":"Barbour","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics, Medicine, and Ecology and Evolutionary Biology, University of California, Irvine, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04945.x"},{"key":"e_1_3_3_3_2","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour AG","year":"1984","unstructured":"BarbourAG. 1984. 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