{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:43Z","timestamp":1791942403604,"version":"4.3.4"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1970,2,1]],"date-time":"1970-02-01T00:00:00Z","timestamp":2678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1970,2]]},"abstract":"\n Hot phenol-water extraction of smooth\n Brucella abortus<\/jats:italic>\n and\n B. melitensis<\/jats:italic>\n cells yielded a toxic fraction which was recovered from the phenol phase (fraction 5). Chemically, fractions 5 from both\n Brucella<\/jats:italic>\n species were lipid-carbohydrate-protein-2 keto-3-deoxyoctulosonic acid complexes which were stable to heat and resistant to Pronase digestion. Electron micrographs of the\n Brucella<\/jats:italic>\n toxins were morphologically indistinguishable from those of enterobacterial endotoxins. Biologically,\n Brucella<\/jats:italic>\n toxins were lethal for mice and immunogenic for rabbits. An intravenous injection of\n Brucella<\/jats:italic>\n toxin induced severe leukopenia with subsequent leukocytosis in mice. Cross-tolerance experiments with mice demonstrated that pretreatment with\n B. abortus<\/jats:italic>\n toxin lessened the hypoferremia produced by challenge with\n Escherichia coli<\/jats:italic>\n endotoxin. Furthermore, fractions 5 from\n B. abortus<\/jats:italic>\n and\n B. melitensis<\/jats:italic>\n were able to form hybrids with\n E. coli<\/jats:italic>\n and\n Salmonella enteritidis<\/jats:italic>\n endotoxins and also with each other. Although\n Brucella<\/jats:italic>\n toxins possess many structural and biological properties in common with endotoxins from the\n Enterobacteriaceae<\/jats:italic>\n , some quantitative differences in their biological potencies were observed.\n Brucella<\/jats:italic>\n toxins were relatively innocuous in tests for pyrogenicity in rabbits and lethality for chick embryos. In nonspecific protection tests,\n Brucella<\/jats:italic>\n toxin had only 1\/75 the potency of\n E. coli<\/jats:italic>\n endotoxin in protecting mice against challenge with virulent\n S. typhi<\/jats:italic>\n . However, on the basis of the data presented and on the work done previously, we concluded that the heat-stable toxins of\n B. abortus<\/jats:italic>\n and\n B. melitensis<\/jats:italic>\n were endotoxins.\n <\/jats:p>","DOI":"10.1128\/iai.1.2.174-182.1970","type":"journal-article","created":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T20:35:01Z","timestamp":1622493301000},"page":"174-182","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Some Structural and Biological Properties of\n Brucella<\/i>\n Endotoxin"],"prefix":"10.1128","volume":"1","author":[{"given":"D.","family":"Leong","sequence":"first","affiliation":[{"name":"Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Milner","sequence":"additional","affiliation":[{"name":"Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Rudbach","sequence":"additional","affiliation":[{"name":"Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. B.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840"},{"name":"University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.1.2.174-182.1970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.1.2.174-182.1970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:47:00Z","timestamp":1646484420000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.1.2.174-182.1970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1970,2]]}},"alternative-id":["10.1128\/iai.1.2.174-182.1970"],"URL":"https:\/\/doi.org\/10.1128\/iai.1.2.174-182.1970","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,2]]},"assertion":[{"value":"1970-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}