{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:08:00Z","timestamp":1791115680205,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1990,3,1]],"date-time":"1990-03-01T00:00:00Z","timestamp":636249600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:p>Despite the recent identification of a number of Mycobacterium leprae proteins, the major immunogenic determinants of this organism remain obscure. We isolated from M. leprae a potent immunostimulatory preparation, designated the MLP fraction, which contains a major protein of 35 kilodaltons (kDa). This protein was precipitated by monoclonal antibody ML03-A1, which recognizes a 35-kDa protein of M. leprae, and by sera obtained from patients with lepromatous leprosy. Neither sera from healthy controls nor sera from patients with pulmonary tuberculosis recognized the 35-kDa protein, and only one of four serum samples from patients with borderline tuberculoid leprosy reacted with this protein. The MLP fraction stimulated T-cell proliferation in patients with leprosy whose T cells proliferate in response to whole M. leprae cells. Apparently, the T-cell epitope associated with MLP is also expressed on M. tuberculosis and M. bovis BCG, since patients with pulmonary tuberculosis and BCG-vaccinated individuals demonstrated significant responses to the MLP fraction. The 35-kDa M. leprae protein, purified to homogeneity in the laboratory of P. J. Brennan, stimulated T-cell proliferative responses in all MLP-responsive subjects. These findings suggest that the 35-kDa protein present in MLP is an immunostimulatory component of M. leprae. In addition to serving as a useful probe for study of the T-cell anergy associated with lepromatous disease, this protein may ultimately be useful as a component of a vaccine designed to provide protection against infection with M. leprae.<\/jats:p>","DOI":"10.1128\/iai.58.3.703-710.1990","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T13:23:33Z","timestamp":1578057813000},"page":"703-710","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Identification of an immunostimulating protein from Mycobacterium leprae"],"prefix":"10.1128","volume":"58","author":[{"given":"N","family":"Mohagheghpour","sequence":"first","affiliation":[{"name":"Department of Pathology, Stanford University, California 94305."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M W","family":"Munn","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University, California 94305."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R H","family":"Gelber","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University, California 94305."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E G","family":"Engleman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University, California 94305."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.58.3.703-710.1990","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.58.3.703-710.1990","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T23:05:14Z","timestamp":1646435114000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.58.3.703-710.1990"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,3]]}},"alternative-id":["10.1128\/iai.58.3.703-710.1990"],"URL":"https:\/\/doi.org\/10.1128\/iai.58.3.703-710.1990","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3]]},"assertion":[{"value":"1990-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}