{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T22:42:12Z","timestamp":1648593732614},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1972,9,1]],"date-time":"1972-09-01T00:00:00Z","timestamp":84153600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1972,9]]},"abstract":"<jats:p>\n            Studies were made on the isolation and identification of the\n            <jats:italic>Rickettsia typhi<\/jats:italic>\n            toxin-neutralizing factor (TNF) previously demonstrated in normal human serum. By means of various methods of separating serum proteins, such as filtration on Sephadex G-200, dextran precipitation, hydroxyapatite chromatography, and ultracentrifugation, TNF was found to be closely associated with purified serum \u03b2-lipoprotein, although no serological relationship with this protein was demonstrated. Lipase as well as trypsin digestion of purified preparations of \u03b2-lipoprotein destroyed the TN activity. No evidence was obtained for an association of TNF with the immunoglobulins or with any serum protein other than \u03b2-lipoprotein. Further studies revealed that (i) serum specimens with TN titers of 1:1024 and others with titers of 1:8 or less contained the same concentration of \u03b2-lipoprotein; (ii) purified preparations of \u03b2-lipoprotein isolated from TNF positive and negative sera, and which had the same protein concentration, differed as much as 250-fold in TN titer; and (iii) the TN activity of a serum could be removed by absorption with antiserum to \u03b2-lipoprotein from a positive donor, but not with antiserum to \u03b2-lipoprotein from a negative donor.\n          <\/jats:p>","DOI":"10.1128\/iai.6.3.232-239.1972","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:16:17Z","timestamp":1578060977000},"page":"232-239","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Murine Typhus Toxin: Studies on Identification of the Neutralizing Factor Present in Normal Human Serum"],"prefix":"10.1128","volume":"6","author":[{"given":"E. John","family":"Bell","sequence":"first","affiliation":[{"name":"National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Munoz","sequence":"additional","affiliation":[{"name":"National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marius","family":"Peacock","sequence":"additional","affiliation":[{"name":"National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Cole","sequence":"additional","affiliation":[{"name":"National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratory, Hamilton, Montana 59840"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.6.3.232-239.1972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.6.3.232-239.1972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:14:04Z","timestamp":1646486044000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.6.3.232-239.1972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1972,9]]}},"alternative-id":["10.1128\/iai.6.3.232-239.1972"],"URL":"https:\/\/doi.org\/10.1128\/iai.6.3.232-239.1972","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,9]]},"assertion":[{"value":"1972-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}