{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T06:11:32Z","timestamp":1793340692090,"version":"4.5.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1995,3,1]],"date-time":"1995-03-01T00:00:00Z","timestamp":794016000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:p>A recombinant plasmid containing ompK36, the gene coding for the Klebsiella pneumoniae outer membrane protein OmpK36, was constructed by transposon mutagenesis and subcloning. Clones were identified in a cosmid library in Escherichia coli on the basis of their reaction with antiserum against the OmpK36 protein and by the presence in gel electrophoretic analysis of a band in E. coli outer membranes migrating with a mobility corresponding to 36 kDa. The ompK36-encoded protein exhibited characteristic properties of porins, such as heat modifiability and resistance to trypsin. The sequence of the gene revealed that OmpK36 is a close relative of the enterobacterial porin family, with a high degree of homology with E. coli OmpC, PhoE, and OmpF. On the basis of the structures of OmpF and PhoE porins, determined previously by X-ray analysis, it appears likely that the three-dimensional structure of OmpK36 also contains the motif of a 16-stranded beta-barrel, with long loops on one end and short turns on the other. Like the OmpC porin from E. coli, OmpK36 contains a long insertion in loop 4. The results of a binding study of complement component C1q to OmpK36 and the analysis of the OmpK36 model suggest that C1q binding sites are covered by the lipopolysaccharide core in the native porin.<\/jats:p>","DOI":"10.1128\/iai.63.3.903-910.1995","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T18:46:20Z","timestamp":1578336380000},"page":"903-910","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":99,"title":["A porin from Klebsiella pneumoniae: sequence homology, three-dimensional model, and complement binding"],"prefix":"10.1128","volume":"63","author":[{"given":"S","family":"Albert\u00ed","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Rodr\u00edquez-Qui\u00f1ones","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Schirmer","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Rummel","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Tom\u00e1s","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J P","family":"Rosenbusch","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V J","family":"Bened\u00ed","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Ambiental, Universidad de las Islas Baleares, Palma de Mallorca, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.63.3.903-910.1995","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.63.3.903-910.1995","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:19:35Z","timestamp":1646468375000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.63.3.903-910.1995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1128\/iai.63.3.903-910.1995"],"URL":"https:\/\/doi.org\/10.1128\/iai.63.3.903-910.1995","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]},"assertion":[{"value":"1995-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}