{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T19:09:24Z","timestamp":1761419364580},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1998,2,1]],"date-time":"1998-02-01T00:00:00Z","timestamp":886291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1998,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The outcome of coccidioidomycosis depends to a large extent on the effectiveness of the T-cell-mediated immune (CMI) response to the fungal pathogen. For this reason, identification of\n            <jats:italic>Coccidioides immitis<\/jats:italic>\n            antigens which stimulate T cells is important for understanding the nature of host defense against the organism and essential for the development of an effective vaccine. Here we describe the immunogenicity of a 48-kDa T-cell-reactive protein (TCRP). The antigen is expressed by parasitic cells and localized in the cytoplasm. It stimulates the proliferative response and production of gamma interferon by T cells of mice immunized with\n            <jats:italic>C. immitis<\/jats:italic>\n            spherules. Specific antibody reactive with the recombinant TCRP (rTCRP) was detected in sera of patients with confirmed coccidioidal infection, and the highest titers of antibody to the recombinant protein correlated with elevated titers to the serodiagnostic complement fixation antigen of\n            <jats:italic>C. immitis<\/jats:italic>\n            . These results suggest that the TCRP is presented to the host during the course of infection. Immunization of BALB\/c and C3H\/HeN mice with the rTCRP affords a modest but significant level of protection against an intraperitoneal challenge with\n            <jats:italic>C. immitis<\/jats:italic>\n            . It is suggested that the rTCRP may be able to contribute to a multicomponent vaccine designed to stimulate CMI response against the parasitic cycle of\n            <jats:italic>C. immitis.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/iai.66.2.424-431.1998","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T15:22:34Z","timestamp":1577805754000},"page":"424-431","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Immunogenicity of a 48-Kilodalton Recombinant T-Cell-Reactive Protein of\n            <i>Coccidioides immitis<\/i>"],"prefix":"10.1128","volume":"66","author":[{"given":"Theo N.","family":"Kirkland","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->VA San Diego Health Care System and Departments of Pathology and Medicine, University of California, San Diego, California 92161,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei Wang","family":"Thomas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 436142"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred","family":"Finley","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->VA San Diego Health Care System and Departments of Pathology and Medicine, University of California, San Diego, California 92161,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Garry T.","family":"Cole","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio 436142"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"235","article-title":"The adjuvant effect of interleukin-12 in a vaccine against Leishmania major.","volume":"163","author":"Afonso L. C.","year":"1995","unstructured":"Afonso L. C. Scharton T. M. Vieira L. Q. Wysocka M. Trinchieri G. Scott P. The adjuvant effect of interleukin-12 in a vaccine against Leishmania major. Science 163 1995 235 237","journal-title":"Science"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.23.3.681-685.1979"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1982.00340160069017"},{"key":"e_1_3_2_5_2","first-page":"281","article-title":"Identification of antigens of Coccidioides immitis which stimulated T lymphocytes.","volume":"24","author":"Cole G. T.","year":"1993","unstructured":"Cole G. T. Kirkland T. N. Identification of antigens of Coccidioides immitis which stimulated T lymphocytes. Arch. Med. Res. 24 1993 281 291","journal-title":"Arch. Med. 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J.\nHarris\nL.\n1985\n281\n333\nPlenum Press\nNew York N.Y","DOI":"10.1007\/978-1-4684-4982-2_12"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.5.1524-1534.1989"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199011000-00006"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90598-6"},{"key":"e_1_3_2_13_2","first-page":"153","article-title":"Coccidioidomycosis.","volume":"159","author":"Galgiani J. N.","year":"1993","unstructured":"Galgiani J. N. Coccidioidomycosis. West. J. Med. 159 1993 153 171","journal-title":"West. J. 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R.\nCole\nG. T.\nStructural studies of Coccidioides immitis Molecular biology of pathogenic fungi. A laboratory manual.\nMaresca\nB.\nKobayashi\nG. S.\n1994\n265\n273\nTelos Press\nNew York N.Y"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.30.6.600"},{"key":"e_1_3_2_28_2","unstructured":"Thomas\nP. W.\nKirkland\nT. N.\nCole\nG. T.\nCharacterization of a T-cell reactive fraction of the parasitic phase of Coccidioides immitis abstr. F-68\nAbstracts of the 97th General Meeting of the American Society for Microbiology 1997.\n1997\n271\nAmerican Society for Microbiology\nWashington D.C"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00250-A"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.66.2.424-431.1998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.66.2.424-431.1998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:21:06Z","timestamp":1646439666000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.66.2.424-431.1998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,2]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,2]]}},"alternative-id":["10.1128\/IAI.66.2.424-431.1998"],"URL":"https:\/\/doi.org\/10.1128\/iai.66.2.424-431.1998","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,2]]},"assertion":[{"value":"1997-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-11-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}