{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:57:24Z","timestamp":1791824244009,"version":"4.3.4"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1999,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In an effort to identify potential cytotoxins expressed by\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            , we have identified a locus that, when mutated in the gonococcus, results in a significant increase in toxicity of the strain to human fallopian tube organ cultures (HFTOC). This locus,\n            <jats:italic>gly1<\/jats:italic>\n            , contains two open reading frames (ORFs) which are likely cotranscribed. ORF1 encodes a polypeptide of 17.8 kDa with a signal sequence that is recognized and processed in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . The 15.6-kDa processed polypeptide has been observed in membrane fractions and filtered spent media from cultures of\n            <jats:italic>E. coli<\/jats:italic>\n            expressing\n            <jats:italic>gly1<\/jats:italic>\n            and in outer membrane preparations of wild-type\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . The\n            <jats:italic>gly1<\/jats:italic>\n            locus is not essential for bacterial survival, and it does not play a detectable role in epithelial cell adhesion, invasion, or intracellular survival. However, a\n            <jats:italic>gly1<\/jats:italic>\n            null mutant causes much more damage to fallopian tube tissues than its isogenic wild-type parent. A strain complemented in\n            <jats:italic>trans<\/jats:italic>\n            for the\n            <jats:italic>gly1<\/jats:italic>\n            mutation showed a level of toxicity to HFTOC similar to the level elicited by the wild-type parent. Taken together, these results indicate an involvement of the\n            <jats:italic>gly1<\/jats:italic>\n            locus in the toxicity of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            to human fallopian tubes.\n          <\/jats:p>","DOI":"10.1128\/iai.67.2.643-652.1999","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T15:32:39Z","timestamp":1577806359000},"page":"643-652","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["<i>Neisseria gonorrhoeae<\/i>\n            Mutants Altered in Toxicity to Human Fallopian Tubes and Molecular Characterization of the Genetic Locus Involved"],"prefix":"10.1128","volume":"67","author":[{"given":"Cindy Grove","family":"Arvidson","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risa","family":"Kirkpatrick","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 627942"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon T.","family":"Witkamp","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Larson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel A.","family":"Schipper","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian S.","family":"Waldbeser","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peadar","family":"O\u2019Gaora","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morris","family":"Cooper","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois 627942"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalene","family":"So","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","unstructured":"Arvidson C. 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