{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:18:42Z","timestamp":1791098322541,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            , the causative agent of plague, two inorganic iron transport systems have been partially characterized. The yersiniabactin (Ybt) system is a siderophore-dependent transport system required for full virulence. Yfe is an ABC transport system that accumulates both iron and manganese. We have identified and cloned a\n            <jats:italic>Y. pestis yfuABC<\/jats:italic>\n            operon. The YfuABC system is a member of the cluster of bacterial ABC iron transporters that include Sfu of\n            <jats:italic>Serratia<\/jats:italic>\n            , Hit of\n            <jats:italic>Haemophilus<\/jats:italic>\n            , and Yfu of\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            . The\n            <jats:italic>Y. pestis<\/jats:italic>\n            KIM6+ system is most homologous to that in\n            <jats:italic>Y. enterocolitica<\/jats:italic>\n            , showing identities of 84% for YfuA (periplasmic binding protein), 87% for YfuB (inner membrane permease), and 75% for YfuC (ATP hydrolase). We constructed a\n            <jats:italic>yfuABC<\/jats:italic>\n            promoter-\n            <jats:italic>lacZ<\/jats:italic>\n            fusion to examine regulation of transcription. This promoter contains a potential Fur binding sequence and is iron and Fur regulated. Significant expression from the\n            <jats:italic>yfuABC<\/jats:italic>\n            promoter occurred during iron-deficient growth conditions. In vitro transcription and translation of a recombinant plasmid encoding\n            <jats:italic>yfuABC<\/jats:italic>\n            indicates that YfuABC proteins are expressed.\n            <jats:italic>Escherichia coli<\/jats:italic>\n            1017 (an enterobactin-deficient mutant) carrying this plasmid was able to grow in an iron-restrictive complex medium. We constructed a deletion encompassing the\n            <jats:italic>yfuABC<\/jats:italic>\n            promoter and most of\n            <jats:italic>yfuA<\/jats:italic>\n            . This mutation was introduced into strains with mutations in Ybt, Yfe, or both systems to examine the role of Yfu in iron acquisition in\n            <jats:italic>Y. pestis<\/jats:italic>\n            . Growth of the\n            <jats:italic>yfu<\/jats:italic>\n            mutants in a deferrated, defined medium (PMH2) at 26 and 37\u00b0C failed to identify a growth or iron transport defect due to the\n            <jats:italic>yfu<\/jats:italic>\n            mutation. Fifty percent lethal dose studies in mice did not demonstrate a role for the Yfu system in mammalian virulence.\n          <\/jats:p>","DOI":"10.1128\/iai.67.5.2829-2837.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:18Z","timestamp":1027763898000},"page":"2829-2837","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":105,"title":["Characterization of the\n            <i>Yersinia pestis<\/i>\n            Yfu ABC Inorganic Iron Transport System"],"prefix":"10.1128","volume":"69","author":[{"given":"Shimei","family":"Gong","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott W.","family":"Bearden","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie A.","family":"Geoffroy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline D.","family":"Fetherston","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Perry","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.572-578.1990"},{"key":"e_1_3_2_4_2","unstructured":"Ausubel\nF. 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