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Members of the gram-negative bacterial genus<jats:italic>Brucella<\/jats:italic>are intracellular pathogens which preferentially invade monocytic cells and develop within these cells. We investigated the effect of<jats:italic>Brucella suis<\/jats:italic>infection on apoptosis of human monocytic phagocytes. The present study provides evidence that<jats:italic>Brucella<\/jats:italic>infection inhibited spontaneously occurring apoptosis in human monocytes. Prevention of monocyte apoptosis was not mediated by<jats:italic>Brucella<\/jats:italic>lipopolysaccharide and required bacterial survival within infected cells. Both invaded and noninvaded cells were protected, indicating that soluble mediators released during infection were involved in the phenomenon. Analysis of<jats:italic>Brucella<\/jats:italic>-infected monocytes revealed specific overexpression of the<jats:italic>A1<\/jats:italic>gene, a member of the<jats:italic>bcl-2<\/jats:italic>family implicated in the survival of hematopoietic cells.<jats:italic>Brucella<\/jats:italic>infection also rendered macrophage-like cells resistant to Fas ligand- or gamma interferon-induced apoptosis, suggesting that<jats:italic>Brucella<\/jats:italic>infection protected host cells from several cytotoxic processes occurring at different steps of the immune response. The present data clearly show that<jats:italic>Brucella suis<\/jats:italic>modulated the monocyte\/macrophage's apoptotic response to the advantage of the pathogen, thus preventing host cell elimination. This might represent a strategy for<jats:italic>Brucella<\/jats:italic>development in infected hosts.<\/jats:p>","DOI":"10.1128\/iai.68.1.342-351.2000","type":"journal-article","created":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T17:57:25Z","timestamp":1241200645000},"page":"342-351","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":207,"title":["In Vitro<i>Brucella suis<\/i>Infection Prevents the Programmed Cell Death of Human Monocytic Cells"],"prefix":"10.1128","volume":"68","author":[{"given":"Antoine","family":"Gross","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->INSERM U431, IFR Euge\u0300ne Bataillon, Universite\u0301 de Montpellier II, 34095 Montpellier Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Terraza","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->INSERM U431, IFR Euge\u0300ne Bataillon, Universite\u0301 de Montpellier II, 34095 Montpellier Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safia","family":"Ouahrani-Bettache","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->INSERM U431, IFR Euge\u0300ne Bataillon, Universite\u0301 de Montpellier II, 34095 Montpellier Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Liautard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->INSERM U431, IFR Euge\u0300ne Bataillon, Universite\u0301 de Montpellier II, 34095 Montpellier Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Dornand","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->INSERM U431, IFR Euge\u0300ne Bataillon, Universite\u0301 de Montpellier II, 34095 Montpellier Cedex 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.35.21878"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.24.11225"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/314844"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)90103-1"},{"key":"e_1_3_2_6_2","unstructured":"Caron E. 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