{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:59:57Z","timestamp":1772816397831,"version":"3.50.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Humans infected with the dimorphic fungus<jats:italic>Blastomyces dermatitidis<\/jats:italic>develop strong T-lymphocyte responses to WI-1, an immunodominant antigen that has been shown to elicit protective immunity in mice. In the present study, the T-cell epitopes of WI-1 and human leukocyte antigen (HLA) restricting elements that display them were investigated. Peripheral blood mononuclear cells (PBMC) from 37 patients with a confirmed history of blastomycosis were tested for a response to WI-1 in primary proliferation assays; PBMC from 35 (95%) responded. Six patients whose PBMC proliferated strongly in response to WI-1 (defined as a stimulation index greater than 50) were tested further for responses to subcloned, recombinant fragments of the antigen. These patients responded chiefly to sequences within the N terminus and the 25-amino-acid tandem repeat. Cloned CD4<jats:sup>+<\/jats:sup>T cells from an infected individual were used to delineate more precisely the peptide epitopes in the fragments and HLA restricting elements that present them. A majority of the T-cell clones recognized an epitope spanning amino acids 149 to 172 within the N terminus, displayed by HLA-DR 15. A minority of the clones, which have been shown to perform a cytolytic function in vitro, recognized an epitope in the tandem repeat displayed by HLA-DPw4, an uncommon restricting element. Tandem repeat epitopes required display by the \u03b2 chain of DPw4 heterodimers. Thus, human T cells with different functions in vitro also recognize distinct regions of WI-1, raising the possibility that HLA restricting elements that present them could modulate immunity during blastomycosis by selection and display of WI-1 peptides.<\/jats:p>","DOI":"10.1128\/iai.68.2.502-510.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"502-510","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["T-Cell Epitopes and Human Leukocyte Antigen Restriction Elements of an Immunodominant Antigen of<i>Blastomyces dermatitidis<\/i>"],"prefix":"10.1128","volume":"68","author":[{"given":"W. L.","family":"Chang","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Internal Medicine, Louisiana State University Medical School, Shreveport, Louisiana 71103,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. G.","family":"Audet","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Pediatrics,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. D.","family":"Aizenstein","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Pediatrics,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. H.","family":"Hogan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Pediatrics,2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. I.","family":"DeMars","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Laboratory of Genetics,3 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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