{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T20:51:52Z","timestamp":1770411112669,"version":"3.49.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Homologous recombination was used to generate a number of mutants of serogroup B\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            B16B6 with the following characteristics: (i) an inability to bind human or porcine transferrin because of loss of both transferrin binding proteins (Tbp) A and B [strain B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>\u2212<\/jats:sup>\n            B\n            <jats:sup>\u2212<\/jats:sup>\n            ] and (ii) an ability to bind porcine transferrin but not human transferrin [strain B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>ap<\/jats:sup>\n            B\n            <jats:sup>ap<\/jats:sup>\n            ] due to replacement of the meningococcal Tbp with the Tbp of\n            <jats:italic>Actinobacillus pleuropneumoniae<\/jats:italic>\n            . During construction of the B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>ap<\/jats:sup>\n            B\n            <jats:sup>ap<\/jats:sup>\n            strain, transformants expressing only TbpA or TbpB of\n            <jats:italic>A. pleuropneumoniae<\/jats:italic>\n            were isolated [strains B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>ap<\/jats:sup>\n            B\n            <jats:sup>\u2212<\/jats:sup>\n            and B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>\u2212<\/jats:sup>\n            B\n            <jats:sup>ap<\/jats:sup>\n            ]. Expression of the\n            <jats:italic>A. pleuropneumoniae<\/jats:italic>\n            Tbp in\n            <jats:italic>N. meningitidis<\/jats:italic>\n            B16B6 was iron regulated and expressed under the control of the meningococcal promoter. The relative abilities of the meningococcal transformants to bind porcine transferrin were in the order B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>ap<\/jats:sup>\n            B\n            <jats:sup>ap<\/jats:sup>\n            &gt; B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>ap<\/jats:sup>\n            B\n            <jats:sup>\u2212<\/jats:sup>\n            &gt; B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>\u2212<\/jats:sup>\n            B\n            <jats:sup>ap<\/jats:sup>\n            . Of these transformants, only B16B6(Str\n            <jats:sup>r<\/jats:sup>\n            )\/tbpA\n            <jats:sup>ap<\/jats:sup>\n            B\n            <jats:sup>ap<\/jats:sup>\n            could grow in the presence of porcine transferrin as the sole iron source, achieving a growth rate similar to that of the B16B6 parent strain in the presence of human transferrin.\n          <\/jats:p>","DOI":"10.1128\/iai.68.2.550-557.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"550-557","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["<i>Neisseria meningitidis<\/i>\n            Expressing Transferrin Binding Proteins of\n            <i>Actinobacillus pleuropneumoniae<\/i>\n            Can Utilize Porcine Transferrin for Growth"],"prefix":"10.1128","volume":"68","author":[{"given":"David J.","family":"Litt","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institute of Infections and Immunity, Queen's Medical Centre, Nottingham, NG7 2UH,1and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen M.","family":"Palmer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institute of Infections and Immunity, Queen's Medical Centre, Nottingham, NG7 2UH,1and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Peter","family":"Borriello","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->PHLS Central Public Health Laboratory, Colindale, London, NW9 5HT,2 United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.11.3162-3170.1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3826-3829.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3421692.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3340269"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1139\/m86-007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1139\/m76-120"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.24.3.330-332.1986"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5381914.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.8.2448-2450.1993"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00710.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01320.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.5.1437-1444.1996"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.13.6127"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1997.0174"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10029-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00692.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-10-2405"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.4.1201-1210.1995"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10025-1"},{"key":"e_1_3_2_21_2","unstructured":"Harlow\nE.\nLane\nD.\nAntibodies: a laboratory manual.\n1988\nCold Spring Harbor Laboratory Press\nCold Spring Harbor N.Y"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-39-1-3"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.29.3.886-891.1980"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01657.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2330-2336.1998"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.364891.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90348-7"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.2501619.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.406943.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(92)90004-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.78.2024.340"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.167.1.138-147.1986"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1997.0156"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-9-757"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-3-257"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(98)00480-7"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.20.6400-6407.1997"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1998.0226"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01635.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1139\/m84-159"},{"key":"e_1_3_2_42_2","unstructured":"Sambrook\nJ.\nFritsch\nE. 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