{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:44:57Z","timestamp":1774565097992,"version":"3.50.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In a search for new skin test reagents specific for tuberculosis, we found that the antigen encoded by gene Rv3874 of\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            elicited delayed-type hypersensitivity in\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            -infected guinea pigs but not in control animals immunized with\n            <jats:italic>Mycobacterium bovis<\/jats:italic>\n            bacillus Calmette-Gue\u0301rin (BCG) or\n            <jats:italic>Mycobacterium avium<\/jats:italic>\n            . The antigen, which was named MTSA-10 (for\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            -specific antigen 10), is a prime candidate for a component of a new tuberculin that will allow discrimination by a skin test of latent\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            infection from vaccination with BCG or from sensitization with environmental, nontuberculous mycobacteria.\n          <\/jats:p>","DOI":"10.1128\/iai.68.2.990-993.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"990-993","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["MTSA-10, the Product of the Rv3874 Gene of\n            <i>Mycobacterium tuberculosis<\/i>\n            , Elicits Tuberculosis-Specific, Delayed-Type Hypersensitivity in Guinea Pigs"],"prefix":"10.1128","volume":"68","author":[{"given":"Roberto","family":"Colangeli","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Public Health Research Institute, New York, New York 100161;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John S.","family":"Spencer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Colorado State University, Fort Collins, Colorado 805232; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Bifani","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Public Health Research Institute, New York, New York 100161;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Williams","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Amersham-Pharmacia Biotech, Piscataway, New Jersey 08855-13273"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin","family":"Lyashchenko","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Public Health Research Institute, New York, New York 100161;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc A.","family":"Keen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Colorado State University, Fort Collins, Colorado 805232; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Preston J.","family":"Hill","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Colorado State University, Fort Collins, Colorado 805232; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Belisle","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Colorado State University, Fort Collins, Colorado 805232; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Laura","family":"Gennaro","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Public Health Research Institute, New York, New York 100161;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"issue":"11","key":"e_1_3_2_2_2","first-page":"18","article-title":"Screening for tuberculosis and tuberculosis infection in high-risk populations. 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