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To monitor the intracellular transport of<jats:italic>B. abortus<\/jats:italic>in macrophages, the kinetics of fusion of phagosomes with preformed lysosomes labeled with colloidal gold particles was observed by electron microscopy. The results indicated that phagosomes containing live<jats:italic>B. abortus<\/jats:italic>were reluctant to fuse with lysosomes. Furthermore, newly endocytosed material was not incorporated into these phagosomes. These observations indicate that the bacteria strongly affect the normal maturation process of macrophage phagosomes. However, after overnight incubation, a significant percentage of the microorganisms were found in large phagosomes containing gold particles, resembling phagolysosomes. Most of the<jats:italic>Brucella<\/jats:italic>bacteria present in phagolysosomes were not morphologically altered, suggesting that they can also resist the harsh conditions prevalent in this compartment. About 50% colocalization of<jats:italic>B. abortus<\/jats:italic>with LysoSensor, a weak base that accumulates in acidic compartments, was observed, indicating that the<jats:italic>B. abortus<\/jats:italic>bacteria do not prevent phagosome acidification. In contrast to what has been described for HeLa cells, only a minor percentage of the microorganisms were found in compartments labeled with monodansylcadaverine, a marker for autophagosomes, and with DiOC6 (3,3\u2032-dihexyloxacarbocyanine iodide), a marker for the endoplasmic reticulum. These results indicate that<jats:italic>B. abortus<\/jats:italic>bacteria alter phagosome maturation in macrophages. However, acidification does occur in these phagosomes, and some of them can eventually mature to phagolysosomes.<\/jats:p>","DOI":"10.1128\/iai.68.7.4255-4263.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"4255-4263","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":124,"title":["Intracellular Trafficking of<i>Brucella abortus<\/i>in J774 Macrophages"],"prefix":"10.1128","volume":"68","author":[{"given":"Graciela N.","family":"Arenas","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Ca\u0301tedra de Microbiolog\u0131\u0301a,1Facultad de Ciencias Me\u0301dicas, Universidad Nacional de Cuyo, Casilla de Correo 56, Mendoza (5500), Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Sandra","family":"Staskevich","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Instituto de Histolog\u0131\u0301a y Embriolog\u0131\u0301a (U.N. 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