{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:14:02Z","timestamp":1790183642607,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2000,8,1]],"date-time":"2000-08-01T00:00:00Z","timestamp":965088000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Proteins belonging to the LraI (for \u201clipoprotein receptor antigen\u201d) family function as adhesins in several streptococci, as a virulence factor for endocarditis in at least one of these species, and potentially as metal transporters in many bacteria. We have identified and characterized the chromosomal locus containing the LraI family gene (designated\n            <jats:italic>sloC<\/jats:italic>\n            ) from\n            <jats:italic>Streptococcus mutans<\/jats:italic>\n            , an agent of dental caries and endocarditis in humans. Northern blot analysis indicated that\n            <jats:italic>sloC<\/jats:italic>\n            is cotranscribed with three other genes. As with other LraI operons, the\n            <jats:italic>sloA<\/jats:italic>\n            and\n            <jats:italic>sloB<\/jats:italic>\n            genes apparently encode components of an ATP-binding cassette transport system. The product of the fourth gene,\n            <jats:italic>sloR<\/jats:italic>\n            , has homology to the metal-dependent regulator from\n            <jats:italic>Corynebacterium diphtheriae<\/jats:italic>\n            , DtxR. A potential binding site for SloR was identified upstream from the\n            <jats:italic>sloABCR<\/jats:italic>\n            operon and was conserved upstream from LraI operons in several other streptococci. Potential SloR homologs were identified in the unfinished genomic sequences from two of these,\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            and\n            <jats:italic>S. pyogenes<\/jats:italic>\n            . Mutagenesis of\n            <jats:italic>sloC<\/jats:italic>\n            in\n            <jats:italic>S. mutans<\/jats:italic>\n            resulted in apparent loss of expression of the entire operon as assessed by Northern blot analysis. The\n            <jats:italic>sloC<\/jats:italic>\n            mutant was indistinguishable from its wild-type parent in a gnotobiotic rat model of caries but was significantly less virulent in a rat model of endocarditis. Virulence for endocarditis was restored by correction of the\n            <jats:italic>sloC<\/jats:italic>\n            mutation but not by provision of the\n            <jats:italic>sloC<\/jats:italic>\n            gene in\n            <jats:italic>trans<\/jats:italic>\n            , suggesting that virulence requires the expression of other genes in the\n            <jats:italic>sloC<\/jats:italic>\n            operon.\n          <\/jats:p>","DOI":"10.1128\/iai.68.8.4441-4451.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"4441-4451","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Genetic Characterization of a\n            <i>Streptococcus mutans<\/i>\n            LraI Family Operon and Role in Virulence"],"prefix":"10.1128","volume":"68","author":[{"given":"Todd","family":"Kitten","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Philips Institute of Oral &amp; Craniofacial Molecular Biology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy L.","family":"Munro","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Adult Health Nursing,2 Virginia Commonwealth University, Richmond, Virginia 23298, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Michalek","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 352943"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis L.","family":"Macrina","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Philips Institute of Oral &amp; Craniofacial Molecular Biology1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00632.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1825-3_153"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07176.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.11.3591-3593.1999"},{"key":"e_1_3_2_7_2","unstructured":"Boos\nW.\nLucht\nJ. 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