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Amino acid microsequencing of p42 revealed that it consists predominantly of schistosome glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). We have expressed SG3PDH in<jats:italic>Escherichia coli<\/jats:italic>and purified the recombinant protein in a soluble and enzymatically active form. Recombinant SG3PDH (rSG3PDH) reacted with human monospecific antibodies to p42. Lymphoproliferation and production of interleukin-4 and gamma interferon (IFN-\u03b3) after in vitro stimulation with rSG3PDH and serum isotype responses to rSG3PDH were examined in individuals with extremes of resistance and susceptibility to reinfection after treatment of previous<jats:italic>S. mansoni<\/jats:italic>or<jats:italic>S. haematobium<\/jats:italic>infection. Lymphoproliferation and IFN-\u03b3 production in response to rSG3PDH and the presence of serum immunoglobulin G1 (IgG1), IgG3, and IgA antibodies to rSG3PDH generally characterized individuals who are resistant to reinfection after chemotherapy. The data indicate that T- and B-cell immune reactivity to rSG3PDH correlates with resistance to reinfection, confirming previous studies identifying SG3PDH as a target of protective immunity in humans, and suggest that SG3PDH should be investigated as a possible vaccine for human schistosomiasis.<\/jats:p>","DOI":"10.1128\/iai.69.1.237-244.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"237-244","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Human T- and B-Cell Responses to<i>Schistosoma mansoni<\/i>Recombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection with<i>S. mansoni<\/i>or<i>Schistosoma haematobium<\/i>after Chemotherapy"],"prefix":"10.1128","volume":"69","author":[{"given":"Rashika","family":"El Ridi","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Zoology Department, Faculty of Science, Cairo University, Cairo,1 and"},{"name":"<!--label omitted: 2-->Biomedical Research Center for Infectious Diseases, The Egyptian Organization for Biological Products and Vaccines,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles B.","family":"Shoemaker","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faten","family":"Farouk","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Biomedical Research Center for Infectious Diseases, The Egyptian Organization for Biological Products and Vaccines,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naglaa H.","family":"El Sherif","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Tropical Medicine, Theodore Bilharz Research Institute,4 Giza, Egypt, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Afifi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Zoology Department, Faculty of Science, Cairo University, Cairo,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(94)E0067-W"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00521-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1996.0113"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1997.4229"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0001-706X(94)90064-7"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90091-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90391-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90086-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90184-L"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(95)00026-7"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60048-2"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.4.1000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/08820139209069383"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.4049\/jimmunol.140.8.2727","article-title":"Human resistance to Schistosoma mansoni is associated with IgG reactivity to a 37-kDa larval surface antigen","volume":"140","author":"Dessein A. 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