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The hypothesis of this study was that activation-induced cell death (AICD) of CD4<jats:sup>+<\/jats:sup>T cells is involved in the immune inflammatory response. This study investigated the dynamics of splenic and granuloma CD4<jats:sup>+<\/jats:sup>Th cell apoptosis and Fas ligand (FasL) expression during the acute and chronic stages of murine schistosomal infection. Enhanced apoptosis of freshly isolated CD4<jats:sup>+<\/jats:sup>Th lymphocytes commenced after egg deposition and persisted during the peak and modulated phases of granuloma formation. After oviposition, CD4<jats:sup>+<\/jats:sup>, CD8<jats:sup>+<\/jats:sup>, and CD19<jats:sup>+<\/jats:sup>splenocytes and granuloma cells expressed elevated levels of FasL but FasL expression declined during the downmodulated stage of infection. In culture, SEA induced splenic and granuloma CD4<jats:sup>+<\/jats:sup>T-cell apoptosis and stimulated expression of FasL on splenic but not granuloma CD4<jats:sup>+<\/jats:sup>T cells, CD8<jats:sup>+<\/jats:sup>T cells, and CD19<jats:sup>+<\/jats:sup>B cells. SEA-stimulated splenocytes and granuloma cells preferentially lysed a Fas-transfected target cell line. Depletion of B cells from SEA-stimulated splenic cultures decreased CD4<jats:sup>+<\/jats:sup>T cell apoptosis. Coculture of purified splenic B cells with CD4<jats:sup>+<\/jats:sup>T cells and adoptive transfer of purified B cells indicated that antigen-stimulated B cells can kill CD4<jats:sup>+<\/jats:sup>Th cells. However, CD4<jats:sup>+<\/jats:sup>T cells were the dominant mediators of apoptosis in the granuloma. This study indicates that AICD is involved in the apoptosis of CD4<jats:sup>+<\/jats:sup>T cells during schistosomal infection.<\/jats:p>","DOI":"10.1128\/iai.69.1.271-280.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:18Z","timestamp":1027763898000},"page":"271-280","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":84,"title":["Soluble Egg Antigen-Stimulated T Helper Lymphocyte Apoptosis and Evidence for Cell Death Mediated by FasL<sup>+<\/sup>T and B Cells during Murine<i>Schistosoma mansoni<\/i>Infection"],"prefix":"10.1128","volume":"69","author":[{"given":"Steven K.","family":"Lundy","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen P.","family":"Lerman","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dov L.","family":"Boros","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.1.71"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00062752-199704010-00006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.3.250"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(11)80450-X"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)80090-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.132.3.488"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.4049\/jimmunol.114.5.1437","article-title":"Spontaneous modulation of granulomatous hypersensitivity in schistosomiasis mansoni","volume":"114","author":"Boros D. 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