{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T00:46:59Z","timestamp":1778978819934,"version":"3.51.4"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In this study, we analyzed whether attachment of\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            vaccine strains to human intestinal epithelial cells can induce an interleukin-8 (IL-8) response. The IL-8 transcripts were detected by PCR amplification of reverse-transcribed mRNA, and the gene product secretion was measured by an enzyme-linked immunosorbent assay. Infection of monolayers of the undifferentiated HT29-18N2 cell line with reactogenic (JBK70 and 81) and nonreactogenic (CVD103HgR and 638) vaccine strains of\n            <jats:italic>V. cholerae<\/jats:italic>\n            resulted in markedly higher IL-8 expression by epithelial cells exposed to reactogenic strains than by cells exposed to the nonreactogenic strains. Additionally, epithelial cells produced IL-8 transcripts following stimulation with cholera vaccine strains in a concentration-dependent manner. These results represent a new insight into the inflammatory component of reactogenicity and could be used as a predictive marker of vaccine reactogenicity prior to human testing.\n          <\/jats:p>","DOI":"10.1128\/iai.69.1.613-616.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"613-616","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Differential Interleukin-8 Response of Intestinal Epithelial Cell Line to Reactogenic and Nonreactogenic Candidate Vaccine Strains of\n            <i>Vibrio cholerae<\/i>"],"prefix":"10.1128","volume":"69","author":[{"given":"Boris L.","family":"Rodr\u0131\u0301guez","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Gene\u0301tica, Centro Nacional de Investigaciones Cient\u0131\u0301ficas,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Rojas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Laboratorio de Farmacolog\u0131\u0301a, Centro de Qu\u0131\u0301mica Farmace\u0301utica,2 La Habana, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Campos","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Gene\u0301tica, Centro Nacional de Investigaciones Cient\u0131\u0301ficas,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talena","family":"Ledon","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Gene\u0301tica, Centro Nacional de Investigaciones Cient\u0131\u0301ficas,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Valle","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Gene\u0301tica, Centro Nacional de Investigaciones Cient\u0131\u0301ficas,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Toledo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Gene\u0301tica, Centro Nacional de Investigaciones Cient\u0131\u0301ficas,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Fando","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Gene\u0301tica, Centro Nacional de Investigaciones Cient\u0131\u0301ficas,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.539-545.1999"},{"key":"e_1_3_2_3_2","first-page":"275","article-title":"Genetic manipulation of Vibrio cholerae for vaccine development: construction of live attenuated El Tor candidate vaccine strains","volume":"27","author":"Benitez J. 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