{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:09:21Z","timestamp":1791223761426,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Numerous studies have reported that asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    , gangliotetraosylceramide, or moieties serve as epithelial cell receptors for\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . Usually this interaction is confirmed with antibodies to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    . However, few, if any, of these reports have evaluated the binding of fresh clinical isolates of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    or the specificity of the antibodies for the asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    antigen. We confirmed that asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    dissolved in dimethyl sulfoxide could be added to the apical membrane of Madin-Darby canine kidney cells growing as a polarized epithelium on Transwell membranes (J. C. Comolli, L. L. Waite, K. E. Mostov, and J. N. Engel, Infect. Immun. 67:3207\u20133214, 1999) and that such treatment enhanced the binding of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    strain PA103. However, no other\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    strain, including eight different clinical isolates, exhibited enhanced binding to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    -treated cells. Studies with commercially available antibodies to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    showed that these preparations had high titers of antibody to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    antigens, including whole cells, purified lipopolysaccharide (LPS), and pili. Inhibition studies showed that adsorption of an antiserum to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    with\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cells could remove the reactivity of antibodies to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    , and adsorption of this serum with asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    removed antibody binding to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    LPS. Antibodies in sera raised to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    were observed to bind to\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cells by immunoelectron microscopy. Antibodies to asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    inhibited formation of a biofilm by\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    in the absence of mammalian cells, indicating a direct inhibition of bacterial cell-cell interactions. These findings demonstrate that asialo-GM\n                    <jats:sub>1<\/jats:sub>\n                    is not a major cellular receptor for clinical isolates of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and that commercially available antibodies raised to this antigen contain high titers of antibody to multiple\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    antigens, which do not interfere with the binding of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    to mammalian cells but possibly interfere with the binding of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cells to each other.\n                  <\/jats:p>","DOI":"10.1128\/iai.69.2.719-729.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:01:19Z","timestamp":1027749679000},"page":"719-729","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Lack of Adherence of Clinical Isolates of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    to Asialo-GM\n                    <sub>1<\/sub>\n                    on Epithelial Cells"],"prefix":"10.1128","volume":"69","author":[{"given":"Torsten H.","family":"Schroeder","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanweer","family":"Zaidi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald B.","family":"Pier","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2130-2136.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.19.2.2889"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115791"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1998.tb01243.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.2.371-376.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1994.1075"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3207-3214.1999"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.12.3500-3507.1994"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.99.13356599"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.5.1582-1588.1996"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2865"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.10.4118-4123.1994"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.43-51.1998"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4891851.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0502.990205"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.10.4572-4579.1994"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00116-9"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.16.5117-5128.1993"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3801"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.12.5164-5173.1993"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3109\/02713689108996341"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1996.46"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.3.1213-1219.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.3019"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(99)00025-6"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)66774-2"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4311793.x"},{"key":"e_1_3_2_29_2","unstructured":"Keleti\nG.\nLederer\nW. H.\nHandbook of micromethods for the biological sciences.\n1974\nVan Nostrand Reinhold Co.\nNew York N.Y"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-98-2-387"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.16.6157"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00348.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1139\/m96-065"},{"key":"e_1_3_2_34_2","first-page":"209","article-title":"Pathogenesis of pneumococcal pneumonia","volume":"14","author":"Novak R.","year":"1999","unstructured":"Novak R. Tuomanen E. Pathogenesis of pneumococcal pneumonia.Semin. Respir. Infect.141999209217","journal-title":"Semin. Respir. Infect."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01062.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.2818-2825.1995"},{"key":"e_1_3_2_37_2","first-page":"1301","article-title":"Fibronectin and \u03b15\u03b21 integrin mediate binding of Pseudomonas aeruginosa to repairing airway epithelium","volume":"13","author":"Roger P.","year":"1999","unstructured":"Roger P. Puchelle E. Bajolet-Laudinat O. Tournier J. M. Debordeaux C. Plotkowski M. C. Cohen J. H. Sheppard D. de Bentzmann S. Fibronectin and \u03b15\u03b21 integrin mediate binding of Pseudomonas aeruginosa to repairing airway epithelium.Eur. Respir. J.13199913011309","journal-title":"Eur. Respir. J."},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.7.2808-2814.1992"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.3.541"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116779"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1993.1073"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(88)90247-5"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1139\/w97-149"},{"key":"e_1_3_2_44_2","first-page":"141","article-title":"Development of an anti-adhesive vaccine for Pseudomonas aeruginosa targeting the C-terminal region of the pilin structural protein","volume":"1","author":"Sheth H. B.","year":"1995","unstructured":"Sheth H. B. Glasier L. M. Ellert N. W. Cachia P. Kohn W. Lee K. K. Paranchych W. Hodges R. S. Irvin R. T. Development of an anti-adhesive vaccine for Pseudomonas aeruginosa targeting the C-terminal region of the pilin structural protein.Biomed. Peptides Prot. Nucleic Acids11995141148","journal-title":"Biomed. Peptides Prot. Nucleic Acids"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00349.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.2950-2957.1995"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.9.3771-3779.1992"},{"key":"e_1_3_2_48_2","first-page":"2096","article-title":"Characterization of pseudomonal adherence to unwounded cornea","volume":"32","author":"Singh A.","year":"1991","unstructured":"Singh A. Hazlett L. Berk R. S. Characterization of pseudomonal adherence to unwounded cornea.Investig. Ophthalmol. Vis. Sci.32199120962104","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.4.1059-1071.1999"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00040a006"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.12.5213-5219.1994"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(95)70549-X"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.1.353-355.1995"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.69.2.719-729.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.69.2.719-729.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T21:04:01Z","timestamp":1646427841000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.69.2.719-729.2001"}},"subtitle":[],"editor":[{"given":"E. I.","family":"Tuomanen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["10.1128\/IAI.69.2.719-729.2001"],"URL":"https:\/\/doi.org\/10.1128\/iai.69.2.719-729.2001","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]},"assertion":[{"value":"2000-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}