{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:04:04Z","timestamp":1791129844082,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            , the etiologic agent of Legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. To evaluate the role of the flagellum in the pathogenesis and ecology of\n            <jats:italic>Legionella<\/jats:italic>\n            , the\n            <jats:italic>flaA<\/jats:italic>\n            gene of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Corby was mutagenized by introduction of a kanamycin resistance cassette. Immunoblots with antiflagellin-specific polyclonal antiserum, electron microscopy, and motility assays confirmed that the specific flagellar mutant\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Corby KH3 was nonflagellated. The redelivery of the intact\n            <jats:italic>flaA<\/jats:italic>\n            gene into the chromosome (\n            <jats:italic>L. pneumophila<\/jats:italic>\n            Corby CD10) completely restored flagellation and motility. Coculture studies showed that the invasion efficiency of the\n            <jats:italic>flaA<\/jats:italic>\n            mutant was moderately reduced in amoebae and severely reduced in HL-60 cells. In contrast, adhesion and the intracellular rate of replication remained unaffected. Taking these results together, we have demonstrated that the flagellum of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            positively affects the establishment of infection by facilitating the encounter of the host cell as well as by enhancing the invasion capacity.\n          <\/jats:p>","DOI":"10.1128\/iai.69.4.2116-2122.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:19Z","timestamp":1027764079000},"page":"2116-2122","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["Flagellum of\n            <i>Legionella pneumophila<\/i>\n            Positively Affects the Early Phase of Infection of Eukaryotic Host Cells"],"prefix":"10.1128","volume":"69","author":[{"given":"Claudia","family":"Dietrich","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, D-97070 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Heuner","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, D-97070 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina C.","family":"Brand","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, D-97070 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u0308rg","family":"Hacker","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, D-97070 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Steinert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Molekulare Infektionsbiologie, Universita\u0308t Wu\u0308rzburg, D-97070 Wu\u0308rzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.9.3127-3133.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02563.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.6.2022-2028.1996"},{"key":"e_1_3_2_5_2","unstructured":"American Type Culture Collection\nCataloge of protists\u2014algae and protozoa\n16th ed.\n1985 Supplement: media formulations. 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