{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T19:37:25Z","timestamp":1771011445945,"version":"3.50.1"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The oral bacterium\n            <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic>\n            is implicated as a causative agent of localized juvenile periodontitis (LJP).\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            is classified into five serotypes (a to e) corresponding to five structurally and antigenically distinct O polysaccharide (O-PS) components of their respective lipopolysaccharide molecules. Serotype b has been reported to be the dominant serotype isolated from LJP patients. We determined the lipopolysaccharide O-PS structure from\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            CU1000, a strain isolated from a 13-year-old African-American female with LJP which had previously been classified as serotype b. The O-PS of strain CU1000 consisted of a trisaccharide repeating unit composed of\n            <jats:sc>l<\/jats:sc>\n            -rhamnose and 2-acetamido-2-deoxy-\n            <jats:sc>d<\/jats:sc>\n            -galactose (molar ratio, 2:1) with the structure \u21922)-\u03b1-\n            <jats:sc>l<\/jats:sc>\n            -Rha\n            <jats:italic>p<\/jats:italic>\n            -(1\u20133)-2-\n            <jats:italic>O<\/jats:italic>\n            -(\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -Gal\n            <jats:italic>p<\/jats:italic>\n            NAc)-\u03b1-\n            <jats:sc>l<\/jats:sc>\n            -Rha\n            <jats:italic>p<\/jats:italic>\n            -(1\u2192. O-PS from strain CU1000 was structurally and antigenically distinct from the O-PS molecules of the five known\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            serotypes. Strain CU1000 was mutagenized with transposon IS\n            <jats:italic>903<\/jats:italic>\n            \u03c6\n            <jats:italic>kan<\/jats:italic>\n            , and three mutants that were deficient in O-PS synthesis were isolated. All three transposon insertions mapped to a single 1-kb region on the chromosome. The DNA sequence of a 13.1-kb region surrounding these transposon insertions contained a cluster of 14 open reading frames that was homologous to gene clusters responsible for the synthesis of\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            serotype b, c, and e O-PS antigens. The CU1000 gene cluster contained two genes that were not present in serotype-specific O-PS antigen clusters of the other five known\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            serotypes. These data indicate that strain CU1000 should be assigned to a new\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            serotype, designated serotype f. A PCR assay using serotype-specific PCR primers showed that 3 out of 20 LJP patients surveyed (15%) harbored\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            strains carrying the serotype f gene cluster. The finding of an\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            serotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted.\n          <\/jats:p>","DOI":"10.1128\/iai.69.9.5375-5384.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"5375-5384","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":129,"title":["Structural and Genetic Analyses of O Polysaccharide from\n            <i>Actinobacillus actinomycetemcomitans<\/i>\n            Serotype f"],"prefix":"10.1128","volume":"69","author":[{"given":"Jeffrey B.","family":"Kaplan","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm B.","family":"Perry","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leann L.","family":"MacLean","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Furgang","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark E.","family":"Wilson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel H.","family":"Fine","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1995.tb00120.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1991.tb00462.x"},{"key":"e_1_3_2_4_2","first-page":"164","article-title":"Correlation of proton chemical shifts by two-dimensional Fourier transform NMR","volume":"42","author":"Bax A.","year":"1981","unstructured":"Bax A. 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