{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T18:42:12Z","timestamp":1649097732178},"reference-count":0,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1973,3,1]],"date-time":"1973-03-01T00:00:00Z","timestamp":99792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1973,3]]},"abstract":"<jats:p>\n            The \u03b2 antigen of\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            has been isolated from the alkaline-extracted gonococcal endotoxin by ion exchange, molecular sieve, and powder block electrophoretic chromatography. Hemagglutination inhibition studies indicate that the preparation is essentially free of common enterobacterial and gonococcal \u03b1 antigen. Analysis of the isolated antigen by immunodiffusion and acrylamide gel electrophoresis reveal only one detectable component. Chemical studies indicate that the antigen is an acidic glycoprotein composed primarily of four major amino acids: alanine, glutamic acid, glycine, and proline. The antigen has an\n            <jats:italic>s<\/jats:italic>\n            <jats:sub>0,w<\/jats:sub>\n            of 8.55, and spectral analysis in the ultraviolet and visible range reveals a single absorption peak at 217 nm.\n          <\/jats:p>","DOI":"10.1128\/iai.7.3.315-321.1973","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:17:43Z","timestamp":1578061063000},"page":"315-321","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Isolation and Characterization of the \u03b2 Antigen of\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"7","author":[{"given":"Michael A.","family":"Apicella","sequence":"first","affiliation":[{"name":"Department of Medicine, State University of New York at Buffalo, Buffalo, New York 14215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James C.","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Medicine, State University of New York at Buffalo, Buffalo, New York 14215"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.7.3.315-321.1973","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.7.3.315-321.1973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:16:32Z","timestamp":1646486192000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.7.3.315-321.1973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1973,3]]}},"alternative-id":["10.1128\/iai.7.3.315-321.1973"],"URL":"https:\/\/doi.org\/10.1128\/iai.7.3.315-321.1973","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,3]]},"assertion":[{"value":"1973-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}