{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T15:01:57Z","timestamp":1761490917268},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1973,4,1]],"date-time":"1973-04-01T00:00:00Z","timestamp":102470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1973,4]]},"abstract":"<jats:p>\n            The macrophage is the main cell participating in chronic inflammation. It contains an acid-acting, cathepsin D-type proteinase with the specificity of pepsin, which may release mediators of the inflammatory process. To find new pharmaceutical inhibitors of this proteinase, we tested a variety of chemical compounds in vitro. For this survey, the possible inhibitor (at a concentration of 0.4 mg\/ml) was assayed with partially purified cathepsin D-type proteinase from beef lung (a macrophage-rich tissue) and hemoglobin as the substrate. Diazophenylbutanone, three acetophenones, two barbiturates, a gold salt, a copper chelate of a substituted nicotinic acid, a hexapeptide containing a\n            <jats:sc>d<\/jats:sc>\n            -amino acid, and Pepstatin inhibited this enzyme; over 200 other potential inhibitors did not. By far the most active and specific inhibitor found to date is Pepstatin, a pentapeptide with two \u03b3-NH linkages, two \u03b2-OH groups, and five branched aliphatic side chains. Banyu Pharmaceutical Co., Tokyo, Japan, produces this nontoxic compound for the treatment of peptic ulcers. In vitro, as little as 4 ng of Pepstatin inhibits the acid-acting cathepsin D-type proteinase purified from beef and rabbit lung as well as the similar proteinase of rabbit peritoneal and pulmonary macrophages.\n          <\/jats:p>","DOI":"10.1128\/iai.7.4.655-665.1973","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T14:18:15Z","timestamp":1578061095000},"page":"655-665","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Inhibition of the Cathepsin D-Type Proteinase of Macrophages by Pepstatin, a Specific Pepsin Inhibitor, and Other Substances"],"prefix":"10.1128","volume":"7","author":[{"given":"Martha H.","family":"McAdoo","sequence":"first","affiliation":[{"name":"Department of Radiological Sciences, School of Hygiene and Public Health, and the Department of Pathology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Arthur M.","family":"Dannenberg","sequence":"additional","affiliation":[{"name":"Department of Radiological Sciences, School of Hygiene and Public Health, and the Department of Pathology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cara J.","family":"Hayes","sequence":"additional","affiliation":[{"name":"Department of Radiological Sciences, School of Hygiene and Public Health, and the Department of Pathology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P.","family":"James","sequence":"additional","affiliation":[{"name":"Department of Radiological Sciences, School of Hygiene and Public Health, and the Department of Pathology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Sanner","sequence":"additional","affiliation":[{"name":"Department of Radiological Sciences, School of Hygiene and Public Health, and the Department of Pathology, School of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.7.4.655-665.1973","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.7.4.655-665.1973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T20:28:20Z","timestamp":1646425700000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.7.4.655-665.1973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1973,4]]}},"alternative-id":["10.1128\/iai.7.4.655-665.1973"],"URL":"https:\/\/doi.org\/10.1128\/iai.7.4.655-665.1973","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,4]]},"assertion":[{"value":"1973-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}